Abstract
GEL electrophoresis separates RNA fractions with higher resolution than the commonly used techniques of column ehromatography and sucrose density gradient centrifugation1–3. Filtration in gel electrophoresis of RNA is well documented2,4–6 and several electrophoretic media have been examined: agar1,7, agarose3, starch8 and polyacrylamide (refs. 4 and 6 and unpublished work of U. Grassbach and I. B. Weinstein). For the microanalysis of RNA base composition9, electrophoresis can be scaled down over a wide range by adopting a system in which the overall dimensions are reduced, the diffusion is restricted by greatly increased viscosity and the migration rate is still sufficient because of a very steep voltage gradient. In spite of such a high field strength there is no problem of inadequate cooling because there is a favourable ratio of surface to volume. We report here that gel electrophoresis can be similarly modified, and describe a procedure which permits fractionation of native RNA in amounts of 10−9 to 10−10 g.
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DANEHOLT, B., RINGBORG, U., EGYHAZY, E. et al. Microelectrophoretic Technique for Fractionation of RNA. Nature 218, 292–293 (1968). https://doi.org/10.1038/218292a0
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DOI: https://doi.org/10.1038/218292a0
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