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Cell Line Models for T Leukemia

Development and characterization of T cell leukemia cell lines established from SCL/LMO1 double transgenic mice

Leukemia volume 15, pages 141–147 (2001)Cite this article

Abstract

We have established a panel of nine immortal cell lines from T cell malignancies which arose in mice transgenic for the SCL and LMO1 genes. Cells from the primary malignancies initially grew very slowly in vitro, loosely attached to a stromal layer, before gaining the ability to proliferate independently. Upon gaining the ability to proliferate in the absence of a stromal layer, these cell lines grew rapidly, doubling every 14–23 h, to a very high density, approaching 107 cells/ml. Whereas the tumors which arise in SCL/LMO1 double transgenic mice are typically diploid or pseudodiploid, the cell lines were all grossly aneuploid, suggesting the possibility that additional genetic events were selected for in vitro. Given that SCL and LMO1 gene activation are both commonly seen in human patients with T cell acute lymphoblastic leukemia, these cell lines may be a useful in vitro model for the human disease.

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Acknowledgements

We would like to thank Drs Ken Gross, Warren Pear, Armelle Regnault and Ilan Kirsch for insightful discussions of these cell lines, the RPCI Biopolymer facility for DNA sequence analysis, and the RPCI Flow Cytometry facility for assistance with immunophenotype analysis. A portion of this work was supported by NIH grants CA16056 and CA6333 and a Leukemia Society of America Scholar grant to PDA.

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Authors and Affiliations

  1. Department of Cancer Genetics, Roswell Park Cancer Institute, USA

    DS Chervinsky & X-F Zhao

  2. Department of Immunology, Roswell Park Cancer Institute, USA

    DH Lam

  3. Genetics Department, Division of Clinical Sciences, Medicine Branch, National Cancer Institute, Gaithersburg, MD, USA

    DH Lam, MP Melman & PD Aplan

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  1. DS Chervinsky
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  2. DH Lam
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  3. X-F Zhao
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Chervinsky, D., Lam, D., Zhao, XF. et al. Development and characterization of T cell leukemia cell lines established from SCL/LMO1 double transgenic mice. Leukemia 15, 141–147 (2001). https://doi.org/10.1038/sj.leu.2401997

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