Table 3 Comparison between different buffers and pressure-cooking times

PC minutes	Case of ALCL (ALK+)	EDTA, pH 8	Tris-EDTA, pH 9	Citrate, pH 6.5
2 min	Nuclear morphology	++	++	+
	FISH intensity	++	++	++
	Immunofluorescence intensity	++	++	±
	Tissue architecture	++	++	+
	Background	No	No	Yes
4 min	Nuclear morphology	+	+	+
	FISH intensity	++	++	++
	Immunofluorescence intensity	+	+	±
	Tissue architecture	++	++	+
	Background	No	No	Yes
8 min	Nuclear morphology	±	±	±
	FISH intensity	±	++	++
	Immunofluorescence intensity	+	+	+
	Tissue architecture	+	++	+
	Background	Yes	No	Yes

++=Good.
+=Sufficient.
±=Not good.
For this experiment, we have used a case of ALCL double stained with CD20 and CD30 monoclonal antibodies using immunofluorescence in combination with the break-apart ALK FISH probe (as described in Table 2).

Quick links

Search