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A microRNA cluster as a target of genomic amplification in malignant lymphoma

Leukemia volume 19, pages 2013–2016 (2005)Cite this article

TO THE EDITOR

MicroRNAs (miRNAs) represent small RNAs (20–24 nt) and non-coding RNAs that perform a multitude of functions.1 MiRNAs display dynamic temporal and spatial expression patterns, where disruption of these patterns might be associated with tumorigenesis. We recently found that C13orf25 is a target gene of 13q31–q32 gain/amplification in malignant lymphomas.2 C13orf25 encodes two variant transcripts by alternative splicing, referred to as C13orf25 transcript variants 1 and 2. Of particular interest are the seven miRNA genes (miR-17, miR-18, miR-19a, miR-19b, miR-20 and miR-92) clustered within the region of C13orf25 transcript variant 2 (C13orf25 v2). The expression of these miRNAs was investigated in the present study in an effort to determine whether these are also overexpressed concomitantly with C13orf25 v2 overexpression using 12 malignant lymphoma cell lines and 21 diffuse large B-cell lymphoma (DLBCL) cases. We recently reported array CGH analysis of genomic gains and losses of 66 cases of DLBCL.3 In all, 21 of the 66 cases were examined for real-time quantitative polymerase chain reaction (PCR) analysis in this study. Of the 21 cases, eight cases showed a genomic gain at 13q, two cases of which showed high copy number gain (amplification) at 13q31. The remaining 13 of the 21 cases showed no copy number changes at 13q.

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  1. Division of Molecular Medicine, Aichi Cancer Center Research Institute, Nagoya, Japan

    H Tagawa & M Seto

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  1. H Tagawa
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  2. M Seto
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Correspondence to H Tagawa.

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Tagawa, H., Seto, M. A microRNA cluster as a target of genomic amplification in malignant lymphoma. Leukemia 19, 2013–2016 (2005). https://doi.org/10.1038/sj.leu.2403942

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