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Affinity labelling the acceptor site of the peptidyl transferase centre of the Escherichia coli ribosome

Nature volume 250, pages 514–516 (1974)Cite this article

Abstract

WE have identified two 50S proteins, L2 and L26-L27, in the peptidyl (P) site of the peptidyl transferase centre of E. coli ribosomes1 BrAc-3H-Phe-tRNAphe, a peptidyl-tRNA affinity analogue, was shown to bind specifically to the P site and covalently react with only L2 and L26-L27. The ability of the same molecules of ribosome-bound BrAcPhe-tRNAphe to participate in dipeptide formation and covalent attachment to ribosomal proteins was the most compelling evidence for functional P site binding.

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Author notes

  1. MARIA PELLEGRINI

    Present address: Department of Chemistry, California Institute of Technology, Pasadena, California

Authors and Affiliations

  1. Department of Biological Chemistry, The Hebrew University of Jerusalem,

    DAN EILAT, MARIA PELLEGRINI, HELEN OEN, NATHAN DE GROOT, YEHUDA LAPIDOT & CHARLES R. CANTOR

  2. Departments of Chemistry and Biological Sciences, Columbia University, New York, New York, 10027

    DAN EILAT, MARIA PELLEGRINI, HELEN OEN, NATHAN DE GROOT, YEHUDA LAPIDOT & CHARLES R. CANTOR

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  1. DAN EILAT
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  2. MARIA PELLEGRINI
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  4. NATHAN DE GROOT
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  5. YEHUDA LAPIDOT
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  6. CHARLES R. CANTOR
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EILAT, D., PELLEGRINI, M., OEN, H. et al. Affinity labelling the acceptor site of the peptidyl transferase centre of the Escherichia coli ribosome. Nature 250, 514–516 (1974). https://doi.org/10.1038/250514a0

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