Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Bacteriophage SP01 regulatory proteins directing late gene transcription in vitro

Abstract

Expression of the temporally defined “late” genes of Bacillus subtilis phage SP01 requires the protein products of regulatory genes 33 and 34. These proteins have now been isolated in pure form. Together with a host factor delta, they act synergistically to direct “late” gene transcription in vitro by core RNA polymerase.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Losick, R., and Pero, J., in RNA polymerase (edit. by Losick, R., and Chamberlin, M.), 227–246 (Cold Spring Harbor Laboratory, New York, 1976).

    Google Scholar 

  2. Fujita, D. J., Ohlsson-Wilhelm, B. M., and Geiduschek, E. P., J. molec. Biol., 57, 301–317 (1971).

    Article  CAS  Google Scholar 

  3. Okubo, S., Yanagida, T., Fujita, D. J., and Ohlsson-Wilhelm, B. M., Biken J., 15, 81–97 (1972).

    CAS  PubMed  Google Scholar 

  4. Fox, T. D., Losick, R., and Pero, J., J. molec. Biol., 101, 427–433 (1976).

    Article  CAS  Google Scholar 

  5. Fox, T. D., and Pero, J., Proc. natn. Acad. Sci. U.S.A., 71, 2761–2765 (1974).

    Article  ADS  CAS  Google Scholar 

  6. Pero, J., Nelson, J., and Fox, T. D., Proc. natn. Acad. Sci. U.S.A., 72, 1589–1593 (1975).

    Article  ADS  CAS  Google Scholar 

  7. Pero, J., Tjian, R., Nelson, J., and Losick, R., Nature, 257, 248–251 (1975).

    Article  ADS  CAS  Google Scholar 

  8. Duffy, J. J., and Geiduschek, E. P., J. biol. Chem., 250, 4530–4541 (1975).

    Article  CAS  Google Scholar 

  9. Whiteley, H. R., Spiegelman, G. B., Lawrie, J. M., and Hiatt, W. R., in RNA polymerase (edit. by Losick, R., and Chamberlin, M.), 587–600 (Cold Spring Harbor Laboratory, New York, 1976).

    Google Scholar 

  10. Fox, T. D., Nature, 748–753 (1976).

    Article  ADS  CAS  Google Scholar 

  11. Shorenstein, R., and Losick, R., J. biol. Chem., 248, 6163–6169 (1973).

    Article  CAS  Google Scholar 

  12. Studier, F. W., J. molec. Biol., 79, 237–248 (1973).

    Article  CAS  Google Scholar 

  13. Zilling, W., Palm, P., and Heil, A., in RNA polymerase (edit. by Losick, R., and Chamberlin, M.), 101–126 (Cold Spring Harbor Laboratory, New York, 1976).

    Google Scholar 

  14. Gage, L. P., and Geiduschek, E. P., J. molec. Biol., 57, 279–300 (1971).

    Article  CAS  Google Scholar 

  15. Tjian, R., Stinchcomb, D., and Losick, R., J. biol. Chem., 250, 8824–8828 (1975).

    Article  CAS  Google Scholar 

  16. Duffy, J. J., Petrusek, R. L., and Geiduschek, E. P., Proc. natn. Acad. Sci. U.S.A., 72, 2366–2370 (1975).

    Article  ADS  CAS  Google Scholar 

  17. Petrusek, R. L., Duffy, J. J., and Geiduschek, E. P., in RNA polymerase (edit. by Losick, R., and Chamberlin, M.), 567–586 (Cold Spring Harbor Laboratory, New York, 1976).

    Google Scholar 

  18. Tjian, R., Pero, J., Losick, R., and Fox, T. D., in Molecular Mechanisms in the Control of Gene Expression (edit. by Nierlich, D. P., Rutter, W. J., and Fox, C. F.), (Academic, New York, in the press).

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tjian, R., Pero, J. Bacteriophage SP01 regulatory proteins directing late gene transcription in vitro. Nature 262, 753–757 (1976). https://doi.org/10.1038/262753a0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/262753a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing