Abstract
BY the application of a wide variety of restriction enzymes and of recently developed DNA sequencing techniques, large segments of the SV40 genome have now been sequenced1,2. As a result, the expression of the viral genetic information can be studied in molecular detail. The major SV40-specific messenger RNA is a polyA-containing, cytoplasmic RNA which sediments at 16S and is only expressed after the onset of DNA replication3. In vitro translation studies have shown that it codes for VP1, the main structural protein of the virion (ref. 4 and A. Smith, personal communication). 16S mRNA hybridises mainly to the SV40 DNA Hind II + III restriction fragments K, F, J and G, which correspond to the region 0.95 to 0.17 on the standard map5,6. The localisation of these restriction fragments and of 16S mRNA is illustrated in Fig. 1.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Van de Voorde, A. et al. in Early Proteins of Oncogenic DNA Viruses (eds May, P., Monier, M. & Weil, R.) 17–30 (INSERM, Paris, 1977).
Dhar, R., Subramanian, K., Pan, J. & Weissman, S. Proc. natn. Acad. Sci. U.S.A. 74, 827–831 (1977).
Weinberg, R., Ben-Ishai, Z. & Newbold, J. J. Virol. 13, 1263–1273 (1974).
Prives, C., Aviv, H., Gilboa, E., Revel, M. & Winocour, E. Cold Spring Harb. Symp. quant. Biol. 39, 309–316 (1974).
May, E., Kopecka, H. & May, P. Nucleic Acids Res. 2, 1995–2005 (1975).
Khoury, G., Carter, B., Ferdinand, F., Howley, P., Brown, M. & Martin, M. J. Virol. 17, 832–840 (1976).
Fiers, W. et al. FEBS Symp. 39, 17–33 (1975).
Van de Voorde, A., Contreras, R., Rogiers, R. & Fiers, W. Cell 9, 117–120 (1976).
Van Heuverswyn, H., Van de Voorde, A. & Fiers, W. Eur. J. Biochem. (in the press)
Zain, B., Weissman, S., Dhar, R. & Pan, J. Nucleic Acids Res. 1, 577–593 (1974).
Haegeman, G. & Fiers, W. J. Virol (in the press).
Groner, Y., Carmi, P. & Aloni, Y. Nucleic Acids Res. 4, 3959–3968 (1977).
Lavi, S. & Shatkin, A. Proc. natn. Acad. Sci. U.S.A. 72, 2012–2016 (1975).
Aloni, Y. FEBS Lett. 54, 363–368 (1975).
Berget, S., Moore, C. & Sharp, P. Proc. natn. Acad. Sci. U.S.A. 74, 3171–3175 (1977).
Klessig, D. Cell 12, 9–21 (1977).
Gilboa, E., Prives, C. & Aviv, H. Biochemistry 14, 4215–4220 (1975).
Volckaert, G., Min Jou, W. & Fiers, W. Analyt. Biochem. 72, 433–446 (1976).
Volckaert, G. & Fiers, W. Analyt. Biochem. 83, 228–239 (1977).
Contreras, R., Rogiers, R. Van de Voorde, A. & Fiers, W. Cell 12, 529–538 (1977).
Fiers, W. et al. Nature (in the press).
Mertz, J. & Berg, P. Proc. natn. Acad. Sci. U.S.A. 71, 4879–4883 (1974).
Shenk, T., Carbon, J. & Berg, P. J. Virol. 18, 664–671 (1976).
Wall, R., Philipson, L. & Darnell, J. Virology 50, 27–34 (1972).
Bachenheimer, S. & Darnell, J. Proc. natn. Acad. Sci. U.S.A. 72, 4445–4449 (1975).
McGuire, P., Swart, C. & Hodge, L. Proc. natn. Acad. Sci. U.S.A. 69, 1578–1582 (1972).
Aloni, Y., Shani, M. & Reuveni, Y. Proc. natn. Acad. Sci. U.S.A. 72, 2587–2591 (1975).
Celma, M., Dhar, R., Pan, J. & Weissman, S. Nucleic Acids Res. 4, 2549–2559 (1977).
Aloni, Y., Dhar, R., Laub, O., Horowitz, M. & Khoury, G. Proc. natn. Acad. Sci. U.S.A. 74, 3686–3690 (1977).
Lavi, S. & Groner, Y. Proc. natn. Acad. Sci. U.S.A. 74, 5323–5327 (1977).
Hsu, M. & Ford, J. Proc. natn. Acad. Sci. U.S.A. 74, 4982–4985 (1977).
Reddy, V., Thimmappaya, B., Dhar, R., Subramanian, K., Zain, B., Pan, J., Celma, C. & Weissman, S. Science (in the press).
De Wachter, R., Merregaert, J., Vandenberghe, A., Contreras, R. & Fiers, W. Eur. J. Biochem. 22, 400–414 (1971).
Van Heuverswyn, H. Van de Voorde, A. & Fiers, W. Nucleic Acids Res. 4, 1015–1024(1977).
Ysebaert, M., Thys, F. Van de Voorde, A. & Fiers, W. Nucleic Acids Res. 3, 3409–3421 (1976).
Ysebaert, M., Van Heuverswyn, H., Van de Voorde, A. & Fiers, W. Eur. J. Biochem. (in the press).
Rogiers, R., Van de Voorde, A., Soeda, E. & Fiers, W. Eur. J. Biochem. (in the press).
Vandenberghe, A., Min Jou, W. & Fiers, W. Proc. natn. Acad. Sci. U.S.A. 72, 2559–2562 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
HAEGEMAN, G., FIERS, W. Evidence for ‘splicing’ of SV40 16S mRNA. Nature 273, 70–73 (1978). https://doi.org/10.1038/273070a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/273070a0
This article is cited by
-
Synthesis of rabbit β-globin in cultured monkey kidney cells following infection with a SV40 β-globin recombinant genome
Nature (1979)
-
Splicing of the late mRNAs of polyoma virus does not occur in the cytoplasm of the infected cell
Nature (1979)
-
Complete nucleotide sequence of SV40 DNA
Nature (1978)
-
Splicing of SV40 late mRNA is a post-transcriptional process
Nature (1978)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.