Abstract
A new approach to cell biology has been created by combining the techniques of micromanipulation of cells, fluorescence spectroscopy and low level light detection. These methods are now being used to study the spatial and temporal distribution, interaction and activity of specific molecules in living cells
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Chambers, R. & Chambers, E. L. Explorations into the Nature of the Living Cell (Harvard University Press, Cambridge, Massachusetts, 1961).
Lorch, I. J. in The Biology of Amoeba (ed. Jeon, K. W.) 1–36 (Academic, New York 1973).
Jeon, K. W., Lorch, I. J., & Danielli, J. F. Science 167, 1626–1627 (1976).
Hiramoto, Y. Expl Cell Res. 27, 416–426 (1962).
Nichols, K. M. & Rikmenspoel, R. J. Cell Sci. 29, 233–247 (1978).
Guilbault, G. G. Practical Fluorescence. Theory, Methods and Techniques (Dekker, New York, 1973).
Fluorescence Spectroscopy (eds Pesce, A. J., Rosen, C. G. & Pasby, T. L.) (Dekker, New York, 1971).
Thaer, A. A. & Sernetz, M. (eds) Fluorescence Techniques in Cell Biology (Springer, Berlin, 1973).
Reynolds, G. T. Quant. Rev. Biophys. 5, 295–347 (1972).
Reynolds, G. T. & Taylor, D. L. BioScience (in the press).
Sedlacek, H. H., Gundlach, H. & Ax, W. Behring, Inst. Mitt 59, 64–70 (1976).
Willingham, M. C. & Pastan, I. Cell 13, 501–507 (1978).
Taylor, D. L., Wang, Yu-Li & Heiple, J. J. Cell Biol. (submitted).
Sengbusch, G. V. & Thaer, A. in Fluorescence Techniques in Cell Biology (eds Thaer, A.A. & Sernetz, M.) 31–39 (Springer, Berlin, 1973).
Taylor, D. L. & Wang, Y.-L. Proc. natn. Acad. Sci. U.S.A. 75, 857–861 (1978).
Wang, Yu-Li & Taylor, D. L. J. Histochem. Cytochem. (submitted).
Wang, Y.-L. & Taylor, D. L. J. Cell Biol. 82, 672–679 (1979).
Cherry, R. J., Cognoli, A., Oppliger, M., Schneider, G. & Semenza, G. Biochemistry 15, 3653–3656 (1976).
Barak, L. S., Yocum, R. R., Nothnagel, E. A. & Webb, W. W. Proc. natn. Acad. Sci. U.S.A. (in the press).
Feramisco, J. R. Proc. natn. Acad. Sci. U.S.A. 76, 3967–3971 (1979).
Kreis, T. E., Winterhalter, K. H. & Birchmeier, W. Proc. natn. Acad. Sci. U.S.A. 76, 3814–3818 (1979).
Waggoner, A. S. A. Rev. Biophys. Bioengng 8, 47–68 (1979).
Diacumakos, E. G. Meth. Cell Biol. 7, 288–311 (1978).
Blinks, J. R. et al. Meth. Enzym. 58, 292–328 (1978).
Rose, B. & Lowenstein, W. R. J. Membrane Biol. 28, 87–199 (1976).
Schlegel, R. A. & Richsteiner, M. C. Meth. Cell Biol. 20, 341–354 (1978).
Tyrrell, D. A., Heath, T. D., Colley, C. M. & Ryman, B. E. Biochim. Biophys. Acta 457, 259–302 (1976).
Gregoriadis, G. New Engl. J. Med. 295, 704–770 (1976).
Yamaizumi, M., Uchida, T., Mekada, E. & Okada, Y. Cell 18, 1009–1014 (1979).
Williamson, R. E. J. Cell Sci. 17, 655–668 (1975).
Miller, M. R., Castellot, J. J. Jr & Pardee, A. B. Expl Cell Res. 120, 421–425 (1979).
Kohen, E., Kohen, C. & Thorell, B. Expl Cell Res. 81, 477–482 (1973).
West, S. S. in Physical Techniques in Biological Research Vol. 3C (ed. Pollister, A. W.) 253–321 (Academic, New York, 1969).
Ploem, J. S., Starke, J. A., De, Bonnet, J. & Wasmund, H. J. Histochem. Cytochem. 22, 668–677 (1974).
Malmstadt, H. V., Franklin, M. L. & Horlick, G. Analyt. Chem. 44, 63A–76A (1972).
Wreford, N. G. M. & Schafield, G. G. J. Microsc. 103, 127–130 (1974).
Rost, F. W. D. & Pearse, A. G. E. J. Microsc. 94, 93–105 (1971).
Cova, S., Prenna, G. & Mazzini, G. Histochem. J. 279–299 (1974).
Axelrod, D., Koppel, D. E., Schlessinger, J., Elson, E. & Webb, W. W. Biophys. J. 16, 1055–1060 (1976).
Elson, H. & Yguerabide, J. J. supramolec. Struct. 12 (in the press).
Smith, B. A. & McConnell, H. M. Proc. natn. Acad. Sci. U.S.A. 75, 2759–2763 (1978).
Koppel, D. E. Biophys. J. 28, 281–292 (1979).
Sheetz, M. P. & Koppel, D. E. Proc. natn. Acad. Sci. U.S.A. 76, 3314–3317 (1979).
Jeon, K. W. & Bell, L. G. E. Expl Cell Res. 33, 531–539 (1964).
Flagg-Newton, J., Simpson, I. & Loewenstein, W. R. Science 205, 404–407 (1979).
Simpson, I., Rose, B. & Loewenstein, W. R. Science 195, 294–296 (1977).
Stacey, D. W. & Allfrey, V. G. J. Cell Biol. 75, 807–817 (1977).
Heiple, J. & Taylor, D. L. J. Cell Biol. (submitted).
Ohkuma, S. & Poole, B. Proc. natn. Acad. Sci. U.S.A. 75, 3327–3331 (1978).
Bittiger, H. & Schnebli, H. P. (eds) Concanavalin A as a Tool (Wiley, New York 1976).
Schlessinger, J. et al. Proc. natn. Acad. Sci. U.S.A. 73, 2407–2413 (1976).
Yahara, I. & Edelman, G. M. Expl Cell Res. 81, 143–155 (1973).
Ryan, G., Borysenko, J. Z. & Karnovsky, M. J. J. Cell Biol. 62, 351–370 (1974).
Oliver, J. M., Ukena, T. E. & Berlin, R. D. Proc. natn. Acad. Sci. U.S.A. 71, 394–398 (1974).
Condeelis, J. R. J. Cell Biol. 80, 751–758 (1979).
Taylor, R. B., Duffus, P. H., Ross, M. C. & DePetris, S. Nature new Biol. 233, 225–229 (1971).
Schreiner, G. F. & Unanue, E. R. Adv. Immun. 24, 38–165 (1976).
Woda, B. A., Yguerabide, J. & Feldman, J. D. J. Immun. 123, 2161–2167 (1979).
Edidin, M. & Fambrough, D. J. Cell Biol. 57, 27–53 (1973).
Keller, P., Person, S. & Snipes, W. J. Cell Sci. 28, 167–177 (1977).
Poo, M-m, Poo, W. J. H. & Lam, J. W. J. Cell Biol. 76, 483–501 (1978).
Clark, J. & Garland, D. J. Cell Biol. 76, 619–627 (1978).
Wulf, E., Deboben, A. Bautz, F. A., Faulstich, H. & Wieland, T. Proc. natn. Acad. Sci. U.S.A. 76, 4498 (1979).
Taylor, D. L. & Condeelis, J. S. Int. Rev. Cytol. 56, 57–144 (1979).
Pastan, I., Webb, W. W. & Elson, E. L. Proc. natn. Acad. Sci. U.S.A. 74, 2909–2913 (1977).
Schlessinger, J., Schechter, Y., Willingham, M. & Pastan, I. Proc. natn. Acad. Sci. U.S.A. 75, 2659–2663 (1978).
Scheckter, Y., Hernaez, L., Schlessinger, J. & Cuatrecasas, P. Nature 278, 835–838 (1979).
Haigler, H., Ash, J. F., Singer, S. J. & Cohen, S. Proc. natn. Acad. Sci. U.S.A. 75, 3317–3321 (1978).
Maxfield, F. R., Schlessinger, J., Schechter, Y., Pastan, I. & Willingham, M. C. Cell 14, 805–810 (1978).
Niedel, J. E., Kahane, I. & Cuatrecasas, P. Science 205, 1412–1414 (1979).
Krieger, M. et al. J. supramolec. Struct. 10, 467–478 (1979).
Axelrod, D. et al. Proc. natn. Acad. Sci. U.S.A. 73, 4594–4598 (1976).
Anderson, M. J. & Cohen, M. W. J. Physiol., Lond. 237, 385–400 (1974).
Block, R. J. J. Cell Biol. 82, 626–643 (1979).
Silverstein, S. C., Steinman, R. M. & Cohn, Z. A. A. Rev. Biochem. 46, 669–722 (1977).
Axelrod, D. Biophys. J. 26, 557–574 (1979).
Nihei, T., Mendelson, R. A. & Botts, J. Biophys. J. 14, 236–242 (1974).
Stryer, L. A. Rev. Biochem. 47, 819–846 (1978).
Wu, C. W. & Stryer, L. Proc. natn. Acad. Sci. U.S.A. 69, 1104–1108 (1972).
Fernandez, S. M. & Berlin, R. D. Nature 264, 411–415 (1976).
Potter, J. D. et al. in Calcium Binding Proteins and Calcium Function (eds Wasserman, R.H. et al.) 239–249 (North-Holland, New York, 1977).
Mabuchi, I. & Okuno, M. J. Cell Biol. 74, 251–263 (1977).
Rungger, D., Rungger-Brändle, E., Chaponnier, C. & Gabbiani, G. Nature 282, 320–321 (1979).
Meeusen, R. L. & Cande, W. Z. J. Cell Biol. 82, 57–65 (1979).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Taylor, D., Wang, YL. Fluorescently labelled molecules as probes of the structure and function of living cells. Nature 284, 405–410 (1980). https://doi.org/10.1038/284405a0
Issue Date:
DOI: https://doi.org/10.1038/284405a0
This article is cited by
-
Live actin brightens up
Nature Reviews Molecular Cell Biology (2010)
-
Widefield fluorescence microscopy with extended resolution
Histochemistry and Cell Biology (2008)
-
Electronic light microscopy: present capabilities and future prospects
Histochemistry and Cell Biology (1995)
-
Dynamic functional and structural analysis of living cells: New tools for vital staining of nuclear DNA and for characterisation of cell motion
Acta Biotheoretica (1995)
-
Cell-to-cell diffusion selectivity in staminal hairs ofSetcreasea purpurea
Protoplasma (1993)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.