Abstract
Direct measurements of free Ca2+ in heart cells are needed for an understanding of the regulation of contractility. We developed and used Ca2+-sensitive microelectrodes with fine tips, stable properties and ample sensitivity to free Ca2+ in the sub-micromolar range. In quiescent ventricular muscle, measurements which passed tests for electrode sealing and cell viability gave a mean free Ca2+ concentration of 0.26 µM. During contractures, we recorded Ca2+ transients rising as high as 10 µM. In studying the effects of catecholamines on free Ca2+ and force, we found evidence that adrenaline can reduce myofibrillar Ca2+ sensitivity in intact heart muscle.
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References
Allen, D. G. & Blinks, J. R. Nature 273, 509–513 (1978).
Allen, D. G. & Kurihara, S. J. Physiol., Lond. 292, 68P–69P (1979).
Wier, W. G. Science 207, 1085–1087 (1980).
Oehme, M., Kessler, M. & Simon, W. Chimia. Aarau 30, 204–206 (1976).
Brown, H. M., Pemberton, J. P. & Owen, J. D. Analytica chim. Acta 85, 261–276 (1976).
Christoffersen, G. R. J. & Simonsen, L. Acta physiol. Scand 101, 492–494 (1977).
Isenberg, G. & Dahl, G. Pflügers Arch. ges. Physiol. 373, R9 (1978).
Sokol, J. H., Lee, C. O. & Lupo, F. J. Biophys. J. 25, 143a (1979).
Chapman, R. A. & Léoty, C. J. Physiol., Lond. 256, 287–314 (1976).
Ammann, D., Güggi, M., Pretsch, E. & Simon, W. Analyt. Lett. 8, 709–720 (1975).
Tsien, R. Y. & Rink, T. J. Biochim. Biophys. Acta 599, 623–638 (1980).
Martell, A. E. & Smith, R. M. Critical Stability Constants Vol. 1 (Plenum, New York, 1974).
Craggs, A., Moody, G. J. & Thomas, J. D. R. Analyst 104, 412–418 (1979).
Ellis, D. J. Physiol., Lond. 273, 211–240 (1977).
Lee, C. O. & Fozzard, H. A. J. gen. Physiol. 65, 695–708 (1975).
Cohen, C. J. & Fozzard, H. A. (in preparation).
Rink, T. J., Tsien, R. Y. & Warner, A. E. Nature 283, 658–660 (1980).
Polimeni, P. I. & Page, E. Circulation Res. 33, 367–374 (1973).
Fabiato, A. & Fabiato, F. J. Physiol., Paris 75, 463–505 (1979).
Matsubara, I. & Millman, B. M. J. molec. Biol. 82, 527–536 (1974).
Moss, R. L., Sollins, M. R. & Julian, F. J. Nature 260, 619–621 (1976).
Lappé, D. L. & Lakatta, E. G. Science 207, 1369–1371 (1980).
Fabiato, A. & Fabiato, F. J. gen. Physiol. 72, 667–699 (1978).
Hibberd, M. G. & Jewell, B. R. J. Physiol., Lond. 290, 30P–31P (1979).
Kavaler, F. & Morad, M. Circulation Res. 18, 492–501 (1966).
Graham, J. A. & Lamb, J. F. J. Physiol., Lond. 197, 479–509 (1968).
Morad, M. & Rolett, E. L. J. Physiol., Lond. 224, 537–558 (1972).
Meinertz, T., Nawrath, H. & Scholz, J. Naunyn-Schmiedebergs Archs Pharmak. 293, 129–137 (1976).
McAfee, D. A., Whiting, G. J. & Siegel, B. J. molec. cell. Cardiol. 10, 705–716 (1978).
Tsien, R. W. Adv. Cyclic Nucleotide Res. 8, 363–420 (1977).
Kirchberger, M., Tada, M. & Katz, A. M. J. biol. Chem. 249, 6166–6173 (1974).
LaRaia, R. J. & Morkin, E. Circulation Res. 35, 298–306 (1974).
Ray, K. & England, P. FEBS Lett. 70, 11–17 (1976).
Cole, H., Frearson, N., Moir, A., Perry, S. J. & Solaro, R. J. Recent Adv. Stud. cardiac Struct. Metab. 11, 111–119 (1978).
McClellan, G. B. & Winegrad, S. J. gen. Physiol. 72, 737–764 (1978).
Holroyde, M. J., Howe, E. & Solaro, R. J. Biochim. biophys. Acta 586, 63–69 (1979).
Weber, A. & Herz, R. J. gen. Physiol. 52, 750–759 (1968).
Coray, A., Fry, C. H., Hess, P., McGuigan, J. S. & Weingart, R. J. Physiol., Lond. 305, 60P–61P (1980).
Uhm, D. Y., Lee, C. O. & Dresdner, K. Fedn Proc. 39, 967 (1980).
Brandt, P. W. & Hibberd, M. G. J. Physiol., Lond. 258, 76P (1976).
Lindley, B. D. & Godt, R. E. Fedn Proc. 39, 580 (1980).
Allen, D. G., Blinks, J. R. & Prendergast, F. Science 195, 996–998 (1977).
Solaro, R. J., Wise, R. M., Shiner, J. S. & Briggs, F. N. Circulation Res. 34, 525–530 (1974).
Fabiato, A. & Fabiato, F. J. Physiol., Lond. 249, 469–495 (1975).
Kerrick, W. G. L. & Donaldson, S. K. B. Pflügers Arch. ges. Physiol. 358, 195–201 (1975).
Endo, M. & Kitizawa, T. in Biophysical Aspects of Cardiac Muscle (ed. Morad, M.) (Academic, New York, 1978).
Lee, D. O., Uhm, D. Y. & Dresdner, K. Science 209, 699–701 (1980).
O'Doherty, J. O., Youmans, S. J., Armstrong, W. McD., & Start, R. J. Science 209, 510–513 (1980).
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Marban, E., Rink, T., Tsien, R. et al. Free calcium in heart muscle at rest and during contraction measured with Ca2+-sensitive microelectrodes. Nature 286, 845–850 (1980). https://doi.org/10.1038/286845a0
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DOI: https://doi.org/10.1038/286845a0
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