Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

A ribosomal protein mediates eIF-2 phosphorylation by interferon-induced kinase

Nature volume 287, pages 65–67 (1980)Cite this article

Abstract

We have previously reported that treatment of mouse L cells with interferon (IFN) induces a reduction of initiation factor (eIF-2) activity (ternary complex formation of eIF-2 with Met-tRNAf and GTP)1, and that the reduction of eIF-2 activity involves the phosphorylation of eIF-2 by an IFN-induced protein kinase2. In addition, we considered that the reduction of eIF-2 activity induced by the kinase (cyclic AMP-independent protein kinase) may be accompanied by the phosphorylation of some intermediary factor(s)2. This possibility was supported by early reports that at least two ribosomal proteins (of molecular weights (MWs) 35,000–38,000 and 64,000–67,000) of mouse L cells3–5 and cellular proteins of Ehrlich ascites tumour cells6 are highly phosphorylated by IFN treatment. Recently, we and others have purified IFN-induced protein kinases from different cells treated with IFN2,3,7–9. These purified kinases show several similarities. The reports suggested that the IFN-induced kinase may be responsible for the phosphorylation of the ribosomal proteins (MWs 35,000–38,000 and 65,000–67,000) and that the phosphorylation of these proteins by the kinase may be associated with the reduced eIF-2 activity in IFN-treated cells2–4,8,9. A similar inhibitory mechanism of eIF-2 activity has been demonstrated in rabbit reticulocyte lysates10–15. The present study was undertaken to purify a ribosomal protein [MW 65,000 (65K)] from mouse L cells and to examine the effect of the protein on eIF-2 activity, and also on the binding of the ternary complex to the 40S ribosomal subunit. We found that the eIF-2 activity was greatly reduced when both eIF-2 and the ribosomal protein were incubated with IFN-induced kinase in vitro. Available evidence suggests that the inhibitory mechanism of eIF-2 activity induced by the kinase in vitro may involve phosphorylation of the ribosomal proteins [a small subunit (MW 38,000) of eIF-2 and 65K ribosomal protein] by the IFN-induced kinase.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Ohtsuki, K., Dianzani, F. & Baron, S. Nature 269, 536–538 (1977).

    Article  ADS  CAS  Google Scholar 

  2. Ohtsuki, K. & Baron, S. J. Biochem. Tokyo 85, 1495–1502 (1979).

    Article  CAS  Google Scholar 

  3. Zilberstein, A., Federman, R., Shulman, L. & Revel, M. FEBS Lett. 68, 119–124 (1976).

    Article  CAS  Google Scholar 

  4. Zilberstein, A., Kimchi, A., Schmidt, A. & Revel, M. Proc. natn. Acad. Sci. U.S.A. 75, 4734–4738 (1978).

    Article  ADS  CAS  Google Scholar 

  5. Gupta, S. J. Virol. 29, 301–311 (1979).

    CAS  PubMed  PubMed Central  Google Scholar 

  6. Lebleu, B., Sen, G. C., Shaila, S., Cabrer, B. & Lengyel, P. Proc. natn. Acad. Sci. U.S.A. 73, 3107–3111 (1976).

    Article  ADS  CAS  Google Scholar 

  7. Sen, G. C., Taira, H. & Lengyel, P. J. biol. Chem. 253, 5915–5921 (1978).

    CAS  PubMed  Google Scholar 

  8. Baglioni, C. Cell 17, 255–264 (1979).

    Article  CAS  Google Scholar 

  9. Kimchi, A., Zilberstein, A., Schmidt, A., Shulman, L. & Revel, M. J. biol. Chem. 254, 9846–9853 (1979).

    CAS  PubMed  Google Scholar 

  10. Datt, A., Haro, C. D., Sierra, J. M. & Ochoa, S. Proc. natn. Acad. Sci. U.S.A. 74, 1463–1467 (1977).

    Article  ADS  Google Scholar 

  11. Datt, A., Haro, C. D. & Ochoa, S. Proc. natn. Acad. Sci. U.S.A. 75, 1148–1152 (1978).

    Article  ADS  Google Scholar 

  12. Lenz, J. R. & Baglioni, C. J. biol. Chem. 253, 4219–4223 (1978).

    CAS  PubMed  Google Scholar 

  13. Petryshyn, R., Trachsel, H. & London, I. M. Proc. natn. Acad. Sci. U.S.A. 76, 1575–1579 (1979).

    Article  ADS  CAS  Google Scholar 

  14. Ernst, V., Levin, D. H. & London, I. M. Proc. natn. Acad. Sci. U.S.A. 76, 2118–2122 (1979).

    Article  ADS  CAS  Google Scholar 

  15. Haro, D. C. & Ochoa, S. Proc. natn. Acad. Sci. U.S.A. 76, 1741–1745 (1979).

    Article  ADS  Google Scholar 

  16. Weber, K. & Osborn, M. J. biol. Chem. 244, 4406–4412 (1969).

    CAS  Google Scholar 

  17. Farrell, P. J., Balkow, K., Hunt, T. & Jackson, R. Cell 11, 187–200 (1977).

    Article  CAS  Google Scholar 

  18. Ohtsuki, K. J. clin. Oncol. Hemat. 10 (in the press).

  19. Tahara, S. M. et al. Proc. natn. Acad. Sci. U.S.A. 75, 789–793 (1978).

    Article  ADS  CAS  Google Scholar 

  20. Trachsel, H. & Staehelin, T. Proc. natn. Acad. Sci. U.S.A. 75, 204–208 (1978).

    Article  ADS  CAS  Google Scholar 

  21. Kozak, M. Cell 15, 1109–1123 (1978).

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Samuel Baron: Department of Microbiology, University of Texas Medical Branch Galveston, Texas 77550

Authors and Affiliations

  1. Department of Bacteriology, Tohoku University School of Medicine, Sendai, 980, Japan

    Kenzo Ohtsuki, Masataka Nakamura, Tsuneaki Koike, Nakao Ishida & Samuel Baron

Authors
  1. Kenzo Ohtsuki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Masataka Nakamura
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tsuneaki Koike
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Nakao Ishida
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Samuel Baron
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ohtsuki, K., Nakamura, M., Koike, T. et al. A ribosomal protein mediates eIF-2 phosphorylation by interferon-induced kinase. Nature 287, 65–67 (1980). https://doi.org/10.1038/287065a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/287065a0

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing