Abstract
The human fibroblast Interferon gene was inserted in a thermoinducible expression plasmid under control of the phage lambda PL promotor. The primary translation products predicted on the basis of the plasmid constructions were hybrid proteins starting with β-lactamase or phage MS2 polymerase information followed by the total preinterferon. On induction, antiviral activity, whose physico-chemical, immunological and biological characteristics closely corresponded to those of authentic human fibroblast Interferon, was synthesized. Processing to a size compatible with mature but unglycosylated authentic product was observed.
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References
Stewart, W. E. II The Interferon System (Springer, Berlin, 1979).
Gresser, I. & Tovey, M. G. Biochim. biophys. Acta 516, 231–247 (1978).
Bloom, B. R. Nature 284, 593–595 (1980).
Desmyter, J. et al. Lancet ii, 645–647 (1976).
Horoszewicz, J. S. et al. Cancer Treat. Rep. 62, 1899–1906 (1978).
Sundmacher, R., Cantell, K., Skoda, K., Hallermann, C. & Neumann-Haefelin, D. Albrecht v. Graefes Arch. Klin. exp. Ophthal. 208, 229–233 (1978).
Dolen, J. G. et al. Am. J. Med. 67, 127–131 (1979).
Treuner, J. et al. Lancet i, 817–818 (1980).
Gutterman, J. U. et al. Ann. int. Med. (in the press).
Derynck, R. et al. Nature 285, 542–547 (1980).
Nagata, S. et al. Nature 284, 316–320 (1980).
Taniguchi, T. et al. Proc. Jap. Acad. 55, Ser B, 464–469 (1979).
Sutcliffe, J. Cold Spring Harb. Symp. quant. Biol. 49, 77–90 (1978).
Devos, R., Van Emmelo, J., Contreras, R. & Fiers, W. J. molec. Biol. 128, 595–619 (1979).
Fiers, W. et al. Nature 260, 500–507 (1976).
Greer, H. Virology 66, 589–604 (1975).
Davey, M. W., Sulkowski, E. & Carter, W. A. J. biol. Chem. 251, 7620–7625 (1976).
Knight, E., Jr Proc. natn. Acad. Sci. U.S.A. 73, 520–523 (1976).
Stewart, W. E. II, De Clercq, E. & De Somer, P. Nature 249, 460–461 (1974).
Kerr, I. M., Brown, R. E. & Hovanessian, A. G. Nature 268, 540–542 (1977).
Neu, H. C. & Heppel, L. A. J. biol. Chem. 240, 3685–3692 (1965).
Havell, E. A., Vilcek, J., Falcoff, E. & Berman, B. Virology 63, 475–483 (1975).
Billiau, A., Joniau, M. & De Somer, P. J. gen. Virol. 19, 1–8, (1973).
Kimchi, A. et al. Proc. natn. Acad. Sci. U.S.A. 76, 3208–3212 (1979).
Minks, M. A., West, D. K., Benvin, S. & Baglioni, C. J. biol. Chem. 254, 10180–10183 (1979).
Wang, R., Shedlarski, J., Farber, M., Kuebbing, D. & Ehrlich, M. Biochim. biophys. Acta (in the press).
Sgaramella, V. & Ehrlich, S. Eur. J. Biochem. 86, 501–537 (1978).
Laemmli, U. K. Nature 227, 680–682 (1970).
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Derynck, R., Remaut, E., Saman, E. et al. Expression of human fibroblast interferon gene in Escherichia coli. Nature 287, 193–197 (1980). https://doi.org/10.1038/287193a0
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DOI: https://doi.org/10.1038/287193a0
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