Abstract
Poliovirus RNA that had been labelled with 125I in the 5′-terminal protein (VPg) was found competent to form an initiation complex of translation in a cell-free reticulocyte lysate. In conditions of ribosome binding, no cleavage occurred between VPg and RNA. We conclude that removal of VPg from poliovirus RNA is not a prerequisite for this RNA to initiate translation in vitro.
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References
Jen, G., Birge, C. H. & Thach, R. E. J. Virol. 27, 640–647 (1978).
Lawrence, C. & Thach, R. E. J. Virol. 14, 598–610 (1974).
Golini, F. et al. Proc. natn. Acad. Sci. U.S.A. 73, 3040–3044 (1976).
Lee, Y. F., Nomoto, A. & Wimmer, E. Prog. Nucleic Acid Res. molec. Biol. 19, 89–96 (1976).
Lee, Y. F., Nomoto, A., Detjen, B. M. & Wimmer, E. Proc. natn. Acad. Sci. U.S.A. 74, 59–63 (1977).
Flanegan, J. B., Pettersson, R. F., Ambros, V., Hewlett, M. J. & Baltimore, D. Proc. natn. Acad. Sci. U.S.A. 74, 961–965 (1977).
Nomoto, A., Detjen, B., Pozzatti, R. & Wimmer, E. Nature 268, 208–213 (1977).
Golini, F., Nomoto, A. & Wimmer, E. Virology 89, 112–118 (1978).
Nomoto, A., Lee, Y. F. & Wimmer, E. Proc. natn. Acad. Sci. U.S.A. 73, 375–380 (1976).
Shih, D. S. et al. Proc. natn. Acad. Sci. U.S.A. 75, 5807–5811 (1978).
Nomoto, A., Kitamura, N., Golini, F. & Wimmer, E. Proc. natn. Acad. Sci. U.S.A. 74, 5345–5349 (1977).
Pérez-Bercoff, R. & Gander, M. FEES Lett. 96, 306–312 (1978).
Ambros, V., Pettersson, R. F. & Baltimore, D. Cell 15, 1439–1446 (1978).
Weber, L. A., Simili, M. & Baglioni, C. in Meth. Enzym. 60, 351–360 (1979).
Stewart-Blair, M. L., Yanowitz, I. S. & Goldberg, I. H. Biochemistry 10, 4198–4206 (1971).
Rothberg, P. G., Harris, T. J. R., Nomoto, A. & Wimmer, E. Proc. natn. Acad. Sci. U.S.A. 75, 4868–4872 (1978).
Wimmer, E. in The Molecular Biology of Picornaviruses (ed. Pérez-Bercoff, R.) 175–190 (Plenum, New York, 1979).
Pettersson, R. F., Ambros, V. & Baltimore, D. Virology 27, 357–366 (1978).
Baltimore, D. in Biochemistry of Viruses (ed. Levy, H. B.) 101–176 (Dekker, New York, 1969).
Pelham, H. R. B. & Jackson, R. J. Eur. J. Biochem. 67, 247–256 (1976).
Darnbrough, C. H., Legon, S., Hunt, T. & Jackson, R. J. J. molec. Biol. 76, 379–403 (1973).
Lee, Y. F., Kitamura, N., Nomoto, A. & Wimmer, E. J. gen. Virol. 44, 311–322 (1979).
Laemmli, U. K. Nature 227, 680–685 (1970).
Bonner, W. M. & Laskey, R. A. Eur. J. Biochem. 46, 83–88 (1974).
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Golini, F., Semler, B., Dorner, A. et al. Protein-linked RNA of poliovirus is competent to form an initiation complex of translation in vitro. Nature 287, 600–603 (1980). https://doi.org/10.1038/287600a0
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DOI: https://doi.org/10.1038/287600a0
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