Abstract
Prostacyclin (PGI2), an unstable metabolite of arachidonic acid synthesized by vascular endothelial and smooth muscle cells, is a potent vasodilator and endogenous inhibitor of platelet aggregation1–10. Regulation of PGI2 synthesis by the vessel wall is not well understood11–16. We have investigated the possibility that a product released from platelet granules during degranulation might modify vessel wall PGI2 biosynthesis. We report here that a non-dialysable, platelet-dependent factor in serum dramatically stimulates PGI2 synthesis by cultured bovine aortic endothelium, aortic smooth muscle, and adrenal capillary endothelium. Platelet-derived growth factor (PDGF), a releasable peptide contained within platelet alpha granules17–20, stimulates PGI2 synthesis by the above cell types as much as 100-fold. The concentrations of PDGF required to produce these effects are below the level reported in normal human serum18. We postulate that in vivo released PDGF may increase vessel wall PGI2 production as part of a negative feedback mechanism controlling platelet aggregation.
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Coughlin, S., Moskowitz, M., Zetter, B. et al. Platelet-dependent stimulation of prostacyclin synthesis by platelet-derived growth factor. Nature 288, 600–602 (1980). https://doi.org/10.1038/288600a0
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DOI: https://doi.org/10.1038/288600a0
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