Abstract
A DNA sequence coding for mature human leukocyte interferon D (LeIF-D) was linked with DNA fragments of the 5′-flanking sequences of the Saccharomyces cerevisiae (yeast) alcohol dehydrogenase I gene in a plasmid capable of autonomous replication and selection in both yeast and Escherichia coli. Yeast cells transformed by these plasmids synthesize up to 1 × 1O6 molecules of biologically active LeIF-D per cell.
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References
Beggs, J. D. Nature 275, 104–109 (1978).
Hinnen, A., Hicks, J. B. & Fink, G. R. Proc. natn. Acad. Sci. U.S.A. 75, 1929–1933 (1978).
Williamson, V. M., Bennetzen, J., Young, E. T., Nasmyth, K. & Hall, B. D. Nature 283, 214–216 (1980).
Petes, T. D. A. Rev. Biochem. 49, 845–876 (1980).
Henikoff, S., Tatchell, K., Hall, B. D. & Nasmyth, K. A. Nature 289, 33–37 (1981).
Beggs, J. D., van den Berg, J., van Ooyen, A. & Weissmann, C. Nature 283, 835–840 (1980).
Goeddel, D. V. et al. Nature 290, 20–26 (1981).
Bennetzen, J. L. & Hall, B. D. J. biol. Chem. (submitted).
Smith, M. et al. Cell 16, 753–761 (1979).
Faye, G., Leung, D. W., Tachell, K., Hall, B. D. & Smith, M. Proc. natn. Acad. Sci. U.S.A. (in the press).
Nagata, S., Mantei, N. & Weissmann, C. Nature 287, 401–408 (1980).
Lawn, R. M. et al. Science 212, 1159–1162 (1981).
Goeddel, D. V. et al. Nature 287, 411–416 (1980).
Stinchcomb, D. T., Struhl, K. & Davis, R. W. Nature 282, 39–43 (1979).
Stewart, W. E. II The Interferon System (Springer, New York, 1979).
Miozzari, G., Neideiberger, P. & Hutter, R. J. Bact. 134, 48–59 (1978).
Kingsman, A. J., Clarke, L., Mortimer, R. & Carbon, J. Gene 7, 141–153 (1979).
Staehlin, T., Hobbs, D. S., Kung, H. & Pestka, S. Proc. natn. Acad. Sci. U.S.A. (in the press).
Kozak, M. Cell 15, 1109–1123 (1978).
Sherman, F., Stewart, J. W. & Schweingruber, A. M. Cell 20, 215–222 (1980).
Seeburg, P. H., Shine, J., Martial, J. A., Baxter, J. D. & Goodman, H. M. Nature 270, 486–494 (1977).
Novick, P., Field, C. & Schekman, R. Cell 21, 205–215 (1980).
Holland, J. P. & Holland, M. J. J. biol. Chem. 255, 2596–2605 (1980).
Ballou, L., Cohen, R. E. & Ballou, C. E. J. biol. Chem. 255, 5986–5991 (1980).
Kuo, S.-C. & Yamamoto, S. Meth. Cell Biol. 11, 169–183 (1975).
Birnboim, H. C. & Doly, J. Nucleic Acids Res. 7, 1513–1523 (1979).
Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 490–565 (1980).
Tschumper, G. & Carbon, J. Gene 10, 157–166 (1980).
Backman, K., Ptashne, M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 73, 4174–4178 (1976).
O'Farrell, P. H. J. biol. Chem. 250, 4007–4021 (1975).
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Hitzeman, R., Hagie, F., Levine, H. et al. Expression of a human gene for interferon in yeast. Nature 293, 717–722 (1981). https://doi.org/10.1038/293717a0
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DOI: https://doi.org/10.1038/293717a0
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