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Synthesis and assembly of hepatitis B virus surface antigen particles in yeast

Nature volume 298, pages 347–350 (1982)Cite this article

Abstract

The surface antigen of hepatitis B virus (HBsAg) has been synthesized in the yeast Saccharomyces cerevisiae by using an expression vector that employs the 5′-flanking region of yeast alcohol dehydrogenase I as a promoter to transcribe surface antigen coding sequences. The protein synthesized in yeast is assembled into particles having properties similar to the 22-nm particles secreted by human cells.

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Biophysics, University of California, San Francisco, California, 94143

    Pablo Valenzuela, Angelica Medina & William J. Rutter

  2. Chiron Corporation, 4560 Horton, Emeryville, California, 94608, USA

    Pablo Valenzuela

  3. Department of Genetics, SK-50, University of Washington, Seattle, Washington, 98195, USA

    Gustav Ammerer & Benjamin D. Hall

Authors
  1. Pablo Valenzuela
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  2. Angelica Medina
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  3. William J. Rutter
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  4. Gustav Ammerer
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  5. Benjamin D. Hall
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Valenzuela, P., Medina, A., Rutter, W. et al. Synthesis and assembly of hepatitis B virus surface antigen particles in yeast. Nature 298, 347–350 (1982). https://doi.org/10.1038/298347a0

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