Abstract
The high mobility group (HMG) proteins1 are a small set of nonhistone chromatin proteins which exhibit preferential affinity for single-stranded DNA2–4. Large portions of the amino acid sequences of calf thymus HMG-1 and HMG-2 have recently been published5. We have analysed that information and describe here an internal sequence homology in the proteins which, coupled with a prediction of secondary structure, strongly suggests that all the high molecular weight HMG proteins (HMG-1, HMG-2 and HMG-E) exist in three domains of comparable lengths. The N-terminal and central domains have homologous sequences and are probably similar in conformation. The C-terminal domain is nonhomologous and highly acidic. We also present results of trypsin digestion experiments which are consistent with the postulated domain structure. We propose that the N-terminal and central domains of the proteins are DNA-binding regions and that the highly acidic C-terminal domain interacts with histones.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Goodwin, G. H., Sanders, C. & Johns, E. W. Eur. J. Biochem. 38, 14–19 (1973).
Bidney, D. L. & Reeck, G. R. Biochem. biophys. Res. Commun. 82, 1211–1218 (1978).
Isackson, P. J., Fishback, J. L., Bidney, D. L. & Reeck, G. R. J. biol. Chem. 254, 5569–5572 (1979).
Isackson, P. J. & Reeck, G. R. Nucleic Acids Res. 9, 3779–3791 (1981).
Walker, J. M., Gooderham, K., Hastings, J. R. B., Mayes, E. & Johns, W. E. FEBS Lett. 122, 264–270 (1980).
de Haën, C., Swanson, E. & Teller, D. C. J. molec. Biol. 106, 639–661 (1976).
Reeck, G. R., Swanson, E. & Teller, D. C. J. molec. Evol. 10, 309–317 (1978).
Sankoff, D. Proc. natn. Acad. Sci. U.S.A. 69, 4–6 (1972).
Sankoff, D. & Cedergren, R. J. J. molec. Biol. 77, 159–164 (1973).
McLachlan, A. D. J. molec. Biol. 61, 409–424 (1971).
de Haën, C., Neurath, H. & Teller, D. C. J. molec. Biol. 92, 225–259 (1975).
Birnbaum, Z. W. Computers and Unconventional Test Statist. tech. Rep. 62 (Office of Naval Research, Contract N-onr-477(38), Seattle, 1973).
Pearson, E. S. & Hartley, H. O. (eds) Biometrika Tables for Statistics Vol. 1, 3rd edn 117 (Cambridge University Press, 1966).
Walker, J. M., Hastings, J. R. B. & Johns, E. W. Nature 271, 281–282 (1978).
Romani, M., Rodman, T. C., Vidali, G. & Bustin, M. J. biol. Chem. 254, 2918–2922.
Cary, P. D. et al. Eur. J. Biochem. 62, 583–590 (1976).
Stein, A., Whitlock, J. P. & Bina, M. Proc. natn. Acad. Sci. U.S.A. 76, 5000–5004 (1979).
Chou, P. Y. & Fasman, G. D. Adv. Enzym. 47, 45–148 (1978).
Baker, C., Isenberg, I., Goodwin, G. H. & Johns, E. W. Biochemistry 15, 1645–1649 (1976).
Isackson, P. J. Thesis, Kansas St. Univ. (1981).
Isackson, P. J., Debold, W. A. & Reeck, G. R. FEBS Lett. 119, 337–342 (1980).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Reeck, G., Isackson, P. & Teller, D. Domain structure in high molecular weight high mobility group nonhistone chromatin proteins. Nature 300, 76–78 (1982). https://doi.org/10.1038/300076a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/300076a0
This article is cited by
-
Sequence of a cDNA Encoding Turtle High Mobility Group 1 Protein
Russian Journal of Genetics (2005)
-
Molecular cloning, expression analysis, and chromosomal localization of mouse Hmg1-containing sequences
Mammalian Genome (1994)
-
Abundance of an mRNA encoding a high mobility group DNA-binding protein is regulated by light and an endogenous rhythm
Plant Molecular Biology (1993)
-
Isolation and patial sequence of bovine cDNA clones for the high-moility-group protein (HMG-1)
Bioscience Reports (1984)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.