Abstract
Human T-cell leukaemia-lymphoma virus (HTLV) was first isolated in the United States from a patient with an aggressive form of cutaneous T-cell lymphoma1 (CTCL) and was later found associated with clusters of adult T-cell leukaemia–lymphomas (ATL) in various parts of the world, including Japan and the Caribbean2–8. Leukaemic cells of the HTLV-positive patients seem to be clonal expansions of single infected cells since the provirus(es) are found at the same sites in a given patient9,10. In avian leukosis virus-induced B-cell lymphomas, the provirus very frequently integrates at several discrete sites in a common domain near the cellular gene, c-myc, that it activates11,12 and it has been speculated that the same would hold true for other chronic leukaemia viruses. We report here that cultured cells from two US patients with CTCL and fresh leukaemia cells of a Japanese patient with ATL contained an HTLV provirus integrated at the same site. In addition, a cord blood T-lymphocyte cell line established by co-cultivation with one of the two HTLV-positive CTCL cell lines13 also contained HTLV provirus contiguous with the same flanking cellular sequences. Ten other HTLV-positive cell samples did not show integration of HTLV at this site, suggesting that there is more than one discrete site of HTLV integration in tumour cells.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Poiesz, B. J. et al. Proc. natn. Acad. Sci. U.S.A. 77, 7415–7419 (1980).
Posner, L. E. et al. J. exp. Med. 154, 333–346 (1981).
Kalyanaraman, V. S., Sarngadharan, M. G., Bunn, P. A., Minna, J. D. & Gallo, R. C. Nature 294, 271–273 (1981).
Kalyanaraman, V. S. et al. Proc. natn. Acad. Sci. U.S.A. 79, 1653–1657 (1982).
Robert-Guroff, M. et al. Science 215, 975–978 (1982).
Catovsky, D. et al. Lancet i, 639–643 (1982).
Blattner, W. A. et al. Int. J. Cancer 30, 257–264 (1982).
Hinuma, Y. et al. Proc. natn. Acad. Sci. U.S.A. 78, 6476–6480 (1981).
Yoshida, M., Miyoshi, I. & Hinuma, Y. Proc. natn. Acad. Sci. U.S.A. 79, 2031–2035 (1982).
Wong-Staal, F. et al. Nature 302, 626–628 (1983).
Neel, B. G., Hayward, W. S., Robinson, H. L., Fang, J. & Astrin, S. M. Cell 23, 323–334 (1981).
Payne, G. S., Bishop, J. M. & Varmus, H. E. Nature 295, 209–213 (1982).
Salahuddin, S. Z., Markham, P. D., Wong-Staal, F., Kalyanaraman, V. S. & Gallo, R. C. Virology (submitted).
Manzari, V. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).
Poiesz, B. J., Ruscetti, F. W., Mier, J. W., Woods, A. M. & Gallo, R. C. Proc. natn. Acad. Sci. U.S.A. 77, 6815–6819 (1980).
Popovic, M. et al. Science 219, 856–859 (1983).
Gallo, R. C. et al. in Modern Trends in Human Leukaemia V (eds Neth, R., Gallo, R. C. & Mannweiler, K.) (Springer, Munich, in the press).
Nusse, R. & Varmus, H. E. Cell 31, 99–109 (1982).
Tsichlis, P. N., Strauss, P. G. & Hu, L. F. Nature 302, 445–449 (1983).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hahn, B., Manzari, V., Colombini, S. et al. Common site of integration of HTLV in cells of three patients with mature T-cell leukaemia-lymphoma. Nature 303, 253–256 (1983). https://doi.org/10.1038/303253a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/303253a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.