Abstract
The three serotypes of poliovirus are members of the picornaviradae, a group of viruses which cause a variety of diseases of considerable importance in man and animals. We have previously used antigenic mutants resistant to neutralizing monoclonal antibodies to identify a single antigenic site for the neutralization of poliovirus type 3 (ref. 1). Evidence based on oligonucleotide mapping suggested that this site corresponded largely to one physicochemical region of the capsid protein viral polypeptide 1 (VP1). We now present conclusive evidence that most of the mutations conferring resistance to neutralization are confined to an eight-amino acid region of VP1, specified by a sequence of viral RNA 277–300 bases from the start of the region coding for VP1. These data strongly suggest that this small region constitutes a major antigenic site involved in virus neutralization and they provide the most detailed information currently available on the antigenic site of a human virus.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Minor, P. D. et al. Nature 301, 674–679 (1983).
Ferguson, M., Minor, P. D., Magrath, D. I., Spitz, M. & Schild, G. C. Rep. Int. Symp. on Poliomyelitis, Washington, 1983 (in the press).
Ferguson, M. et al. Lancet ii, 122–124 (1982).
Minor, P. D. J. gen. Virol. 59, 307–317 (1982).
Caton, A., Brownlee, G. G., Yewdell, J. W. & Gerhard, W. Cell 31, 417–427 (1982).
Cann, A. J. et al. Nucleic Acids Res. (in the press).
Emini, E. A., Jameson, B. A., Lewis, A. J., Larsen, G. R. & Wimmer, E. J. Virol. 43, 997–1005 (1982).
Emini, E. A., Shaw-Yi, Kao, Lewis, A. J. & Wimmer, E. J. Virol. (in the press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Evans, D., Minor, P., Schild, G. et al. Critical role of an eight-amino acid sequence of VP1 in neutralization of poliovirus type 3. Nature 304, 459–462 (1983). https://doi.org/10.1038/304459a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/304459a0
This article is cited by
-
Antigen chimaeras of poliovirus as potential new vaccines
Nature (1988)
-
The sequence of the coxsackievirus A21 polymerase gene indicates a remarkably close relationship to the polioviruses
Archives of Virology (1987)
-
Development of a Genetically–Engineered, Candidate Polio Vaccine Employing the Self–Assembling Properties of the Tobacco Mosaic Virus Coat Protein
Nature Biotechnology (1986)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.