Abstract
Valosin, a novel 25-amino-acid peptide isolated recently from pig intestine1, has several effects on the digestive system of dogs2. We report here that the valosin-specific complementary DNA clone from pigs codes for a polypeptide unlike most precursors of biologically active peptides. The predicted protein lacks a characteristic amino-terminal hydrophobic signal sequence and contains no processing signals of the type acted upon by endopeptidases to generate other active peptides from precursors. Antibodies to synthetic valosin have been used to show that nearly all valosin immunoreactivity is in the cytoplasm and that the protein detected (valosin-containing protein, VCP), although smaller than the predicted product of the cDNA sequence, is much larger than valosin. Valosin-specific messenger RNA is found in extracts from many pig tissues, which contrasts with the restricted occurrence expected of a biologically active peptide. We conclude that valosin is an artefact of the purification procedure and does not occur in vivo.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Schmidt, W. E. et al. FEBS Lett. 191, 264–268 (1985).
Schmidt, W. E. et al. Dig. Dis. Sci. 29, 75S (1984).
Tatemoto, K. & Mutt, V. Proc. natn. Acad. Sci. U.S.A. 75, 4115–4119 (1978).
Tatemoto, K. Proc. natn. Acad. Sci. U.S.A. 79, 2514–2518 (1982).
Tatemoto, K. & Mutt, V. Proc. natn. Acad. Sci. U.S.A. 78, 6603–6607 (1981).
Tatemoto, K., Rökaeus, Å, Jörnvall, H., McDonald, T. J. & Mutt, V. FEBS Lett. 164, 124–128 (1983).
Mutt, V. in Gastrointestinal Hormones (ed. Glass, G. B. J.) 169–180 (Raven, New York, 1980).
Jorpes, J. E., Mutt, V., Magnusson, S. & Steele, B. B. Biochem. biophys. Res. Commun. 9, 275–278 (1962).
Said, S. I. & Mutt, V. Eur. J. Biochem. 28, 199–204 (1972).
Brown, J. C., Cook, M. A. & Oryburgh, J. R. Gastroenterology 62, 401–404 (1972).
Rökaeus, Å, & Brownstein, M. J. Proc. natn. Acad. Sci. U.S.A. 83, 6287–6291 (1986).
Grunstein, M. & Hogness, D. Proc. natn. Acad. Sci. U.S.A. 72, 3961–3965 (1975).
Young, W. S. III, Mezey, E. & Siegel, R. E. Neurosci. Lett. 70, 198–203 (1986).
Von Heijne, G. Nucleic Acids Res. 14, 4683–4690 (1986).
Hopp, T. P. & Woods, K. R. Proc. natn. Acad. Sci. U.S.A. 78, 3824–3828 (1981).
Melton, D. A. et al. Nucleic Acids Res. 12, 7035–7056 (1984).
Pelham, H. R. B. & Jackson, R. J. Eur. J. Biochem. 67, 247–256 (1976).
Kozak, M. Cell 44, 283–292 (1986).
Schally, A. V. et al. Biochem. biophys. Res. Comm. 82, 582–588 (1978).
Kiso, Y. et al. FEBS Lett. 155, 281–284 (1983).
Maniatis, T., Fritsch, E. F. & Sambrook, J. Molecular Cloning; A Laboratory Manual (Cold Spring Harbor Laboratory, New York, 1982).
Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).
Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J. & Rutter, W. J. Biochemistry 18, 5294–5299 (1979).
Feinberg, A. P. & Vogelstein, B. Analyt. Biochem. 137, 266–267 (1984).
O'Farrell, P. J. biol. Chem. 250, 4007–4021 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Koller, K., Brownstein, M. Use of a cDNA clone to identify a supposed precursor protein containing valosin. Nature 325, 542–545 (1987). https://doi.org/10.1038/325542a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/325542a0
This article is cited by
-
Autophagy Dysfunction in ALS: from Transport to Protein Degradation
Journal of Molecular Neuroscience (2022)
-
OsCDC48/48E complex is required for plant survival in rice (Oryza sativa L.)
Plant Molecular Biology (2019)
-
Strategic role of the ubiquitin-dependent segregase p97 (VCP or Cdc48) in DNA replication
Chromosoma (2017)
-
Peroxisome assembly factor–2, a putative ATPase cloned by functional complementation on a peroxisome–deficient mammalian cell mutant
Nature Genetics (1995)
-
Subunits of the regulatory complex of the 26S protease
Molecular Biology Reports (1995)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.