Abstract
KILLER strains of the yeast Kluyveromyces lactis secrete a complex protein toxin, production of which is associated with the presence of two linear double-stranded DNA plasmids, pGKLl and pGKL21. The toxin is encoded by two of the four genes carried by pGKLl2 and is active against yeast from several genera (including Saccharomyces cerevisiae)1, 3. The toxin arrests sensitive yeast cells in the unbudded (G1) phase of the cell cycle4, indicating that it could be used to study stage-specific events occurring in G1. Previous work indicated that K. lactis toxin was a reversible inhibitor of adenylyl cyclase in sensitive S. cerevisiae cells4. Here we report that although the toxin does induce G1 arrest, its effects on S. cerevisiae cells cannot be mediated by inhibition of adenylyl cyclase, and that treated cells never recover from its effects.
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White, J., Butler, A. & Stark, M. Kluyveromyces lactis toxin does not inhibit yeast adenylyl cyclase. Nature 341, 666–668 (1989). https://doi.org/10.1038/341666a0
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DOI: https://doi.org/10.1038/341666a0
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