Abstract
THE L-type voltage-dependent calcium channel is an important link in excitation-contraction coupling of muscle cells1 (reviewed in refs 2 and 3). The channel has two functional characteristics: calcium permeation and receptor sites for calcium antagonists. In skeletal muscle the channel is a complex of five subunits, α1, α2, β, γ and δ (ref. 4). Complementary DNAs to these subunits have been cloned and their amino-acid sequences deduced5–8. The skeletal muscle α1 subunit cDNA expressed in L cells manifests as specific calcium-ion permeation, as well as sensitivity to the three classes of organic calcium-channel blockers9,10. We report here that coexpression of the α1, subunit with other subunits results in significant changes in dihydropyridine binding and gating properties. The available number of drug receptor sites increases 10-fold with an α1 β combination, whereas the affinity of the dihydropyridine binding site remains unchanged. Also, the presence of the β subunit accelerates activation and inactivation kinetics of the calcium-channel current.
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References
Rios, E. & Brum, G. Nature 325, 717–720 (1987).
Hess, P. A. Rev. Neurosci. 13, 337–356 (1990).
Tsien, R. W. & Tsien, R. Y. An. Rev. Cell Biol. 6, 715–760 (1990).
Vaghy, P. L., McKenna, E., Itagaki, K. & Schwartz, A. Trends pharmacol. Sci. 9, 398–402 (1988).
Tanabe, T. et al. Nature 328, 313–318 (1987).
Ellis, S. B. et al. Science 241, 1661–1664 (1988).
Ruth, P. et al. Science 245, 1115–1118 (1989).
Jay, S. D. et al. Science 248, 490–492 (1990).
Perez-Reyes, E. et al. Nature 340, 233–236 (1989).
Kim, H. S. et al. J. biol. Chem. 265, 11858–11863 (1990).
Janis, R. A., Silver, P. J. & Triggle, D. J. Adv. Drug Res. 16, 309–591 (1987).
Lory, P., Rassendren, F. A., Richard, S., Tiaho, F. & Nargeot, J. J. Physiol., Lond. 429, 95–112 (1990).
Caffrey, J. D., Brown, A. M. & Schneider, M. D. Science 236, 570–573 (1987).
Caffrey, J. D., Brown, A. M. & Schneider, M. D. J. Neurosci. 9, 3443–3453 (1989).
Mikami, A. et al. Nature 340, 230–233 (1989).
Biel, M. et al. FEBS Lett. 269, 409–412 (1990).
Mori, Y. et al. Nature 350, 398–402 (1991).
Slish, D. F. et al. FEBS Lett. 250, 509–514 (1989).
Koch, W. J., Ellinor, P. T. & Schwartz, A. J. biol. Chem. 265, 17786–17791 (1990).
Perez-Reyes, E., Wei, X., Castellano, A. & Birnbaumer, L. J. biol. Chem. 265, 20430–20436 (1990).
Pragnell, M., Leveille, C. F., Jay, S. D. & Campbell, K. P. Biophys. J. 59, 390 (1991).
Schwartz, L. M., McCleskey, E. W. & Aimers, W. Nature 314, 747–751 (1985).
McPherson, G. A. KINETIC, EBDA, LIGAND. LOWRY (Elsevier, UK, 1985).
Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).
Sambrook, J., Fritsch, E. F. & Maniatis, T. Molecular Cloning: a Laboratory Manual 2nd edn (Cold Spring Harbor Laboratory, New York, 1989).
Chen, C. & Okayama, H. Molec. cell. Biol. 7, 2745–2752 (1987).
Lee, F., Mulligan, R., Berg, P. & Ringold, G. Nature 294, 228–232 (1981).
Okayama, H. & Berg, P. Molec. cell. Biol. 3, 280–289 (1983).
Hamill, O. P. et al. Pflügers Arch. 391, 85–100 (1981).
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Varadi, G., Lory, P., Schultz, D. et al. Acceleration of activation and inactivation by the β subunit of the skeletal muscle calcium channel. Nature 352, 159–162 (1991). https://doi.org/10.1038/352159a0
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DOI: https://doi.org/10.1038/352159a0
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