New technologies for multiple chemical synthesis of oligonucleotides and stepwise hybridization on a solid-phase support enable the rapid and cost-effective preparation of long duplex DNA regions. Will these new technologies usher in a new era in protein engineering?
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Efficient assembly of very short oligonucleotides using T4 DNA Ligase
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Beattie, K., Fowler, R. Solid-phase gene assembly. Nature 352, 548–549 (1991). https://doi.org/10.1038/352548a0
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DOI: https://doi.org/10.1038/352548a0
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