Abstract
IN many mammalian cells brefeldin A interferes with mechanisms that keep the Golgi apparatus separate from the endoplasmic reticulum1–8. The earliest effect of brefeldin A is release of the coat protein β-COP from the Golgi9–11. This release is blocked by pretreatment with GTP-γS or AIF−4 (ref. 12). The AIF−4 ion activates heterotrimeric G proteins13 but not proteins of the rassuperfamily14, suggesting that a heterotrimeric G protein might control membrane transfer from the endoplasmic reticulum to the Golgi. We report here that mastoparan, a peptide that activates heterotrimeric G proteins15,16, promotes binding ofβ-COP to Golgi membranes in vitro and antagonizes the effect of brefeldin A on β-COP in perforated cells and on isolated Golgi membranes. This inhibition is greatly diminished if cells are pretreated with pertussis toxin before perforation., Thus, a heterotrimeric G protein of the Gi/GO subfamily regulates association of coat components with Golgi membranes.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Misumi, Y., Miki, K., Takatsuki, A., Tamura, G. & Ikehara, Y. J. biol. Chem. 261, 11398–11403 (1986).
Oda, K. et al. FEBS Lett. 214, 135–138 (1987).
Fujiawa, T. Oda, K., Yokota, S., Takatusuki, A. & Ikehara, Y. J. biol. Chem. 263, 18545–18552 (1988).
Doms, R. W., Russ, G. & Yewdell, J. W. J. Cell Biol. 109, 61–72 (1989).
Lippincott-Schwartz, J., Yaun, L. C., Bonifacino, J. S. & Klausner, R. D. Cell 54, 209–220 (1989).
Fujiwara, T., Oda, K. & Ikehara, Y. Cell Struct. Funct. 14, 605–616 (1989).
Ulmer, J. & Palade, G. Proc. natn. Acad. U.S.A. 86, 6992–6996 (1989).
Lippincott-Schwartz, J. et al. Cell 60, 821–836 (1990).
Duden, R. Griffiths, G., Frank, R., Argos, P. & Kreis, T. E. Cell 64, 649–665 (1991).
Serafini, T. et al. Nature 349, 215–220 (1991).
Donaldson, J. G. Lippincott-Schwartz, J., Bloom, G. S., Kreis, T. E. & Klausner, R. D. J. Cell Biol. 111, 2295–2306 (1990).
Donaldson, J. G., Lippincott-Schwartz, J. & Klausner, R. D. J. Cell Biol. 112, 579–578 (1991).
Gilman A. G. A. Rev. Biochem. 56, 615–649 (1987).
Kahn, R. A. J. biol. Chem. 266, 25595–25597 (1991).
Higashijima, T. Uzu, S., Nakajima, T. & Ross, E. M. J. biol. Chem 263, 6491–6494 (1988).
Higashijima, T., Burnier, J. & Ross, E. M. J. biol. Chem. 265, 14176–14186 (1990).
Simons, K. & Virta, H. EMBO J. 6, 2241–2247 (1987).
Ktistakis, N. T., Roth, M. G. & Bloom, G. S. J. Cell Biol. 113, 1009–1023 (1991).
Orci, L. et al. Cell 64, 1183–1195 (1991).
Donaldson, J. G., Kahn, R. A., Lippincott-Schwartz, J. & Klausner, R. D. Science 254, 1197–1199 (1991).
Stow, J. L. et al. J. Cell Biol. 114, 1113–1124 (1991).
Balch, W. E., Dunphy, W. G., Braell, W. A. & Rothman, J. E. Cell 39, 405–416 (1984).
Allan, V. J. & Kreis, T. E. J. Cell Biol. 103, 2229–2239 (1986).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ktistakis, N., Linden, M. & Roth, M. Action of brefeldin A blocked by activation of a pertussis-toxin-sensitive G protein. Nature 356, 344–346 (1992). https://doi.org/10.1038/356344a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/356344a0
This article is cited by
-
Ways of improving precise knock-in by genome-editing technologies
Human Genetics (2019)
-
Regulation of Golgi signaling and trafficking by the KDEL receptor
Histochemistry and Cell Biology (2013)
-
The regulatory domain of protein kinase Cθ localises to the Golgi complex and induces apoptosis in neuroblastoma and Jurkat cells
Cell Death & Differentiation (2003)
-
G proteins and regulation of adenylyl cyclase
Bioscience Reports (1995)
-
Regulation of apical transport in epithelial cells by a Gsclass of heterotrimeric G protein
Nature (1993)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.