Abstract
TWO cytosolic fractions (A and B) fromXenopus oocytes are sufficient to support protein import into the nuclei of digitonin-permeabilized cells1. Fraction A recognizes the nuclear localization sequence (NLS) and binds the import substrate to the nuclear envelope, whereas fraction B mediates the subsequent passage of the bound substrate into the nucleus. Here we report that two interacting components are required for full fraction-B activity, purify one of these components to homogeneity, and show that it is the highly abundant GTP-binding protein Ran (Ras-related nuclear protein)/TC4.
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Moore, M., Blobel, G. The GTP-binding protein Ran/TC4 is required for protein import into the nucleus. Nature 365, 661–663 (1993). https://doi.org/10.1038/365661a0
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DOI: https://doi.org/10.1038/365661a0
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