Abstract
A truncating mutation (C to T transition) at codon 531 of the human protooncogene c-src, possibly accounting for the activation of c-src tyrosine kinase, has been recently identified in a subset of advanced colorectal cancer from North-American patients. However, two subsequent studies have failed to confirm the occurrence of SRC 531 mutation in colorectal cancers from North-European and Asiatic patients, raising the hypothesis that the genetic activation of src in colon cancer might be restricted to patients belonging to specific ethnic groups. We investigated a large series of colorectal cancers from Italian patients (155 cases) with a high prevalence of liver metastasis (43%). Using a PCR-RFLP assay, the occurrence of a SRC 531 mutation was ruled out in all the investigated specimens of primary tumours and/or metastases. Our results demonstrate that SRC Gln531AMB plays no role in the development or in the progression of colorectal cancer among Italian patients. © 2001 Cancer Research Campaign http://www.bjcancer.com
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16 November 2011
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Daigo Y, Furukawa T, Ishiguro H, Fujita M, Sugai S, Nakamori S, Liefers GJ, Tollenaar RAEM, van de Velde CJH and Nakamura Y (1999) Absence of genetic alteration at codon 531 of the human c-src gene in 479 advanced colorectal cancers from Japanese and Caucasian patients. Cancer Res 59: 4222–4224
Irby RB, Mao WN, Coppola D, Kang J, Loubeau JM, Trudeau W, Kael R, Fujita DJ, Jove R and Yeatman TJ (1999) Activating SRC mutation in a subset of advanced human colon cancers. Nat Genet 21: 187–190
Mao W, Irby R, Coppola D, Fu L, Wloch M, Turner J, Yu H, Garcia R, Jove R and Yeatman TJ (1997) Activation of c-Src by receptor tyrosine kinases in human colon cancer cells with high metastatic potential. Oncogene 15: 3083–3090
Ottenhoff-Kalff AE, Rijksen G, van Beurden EA, Hennipman A, Michels AA and Staal GE (1992) Characterization of protein tyrosine kinases from human breast cancer: involvement of the c-src oncogene product. Cancer Res 52: 4773–4778
Talamonti MS, Roh MS, Curley SA and Gallick GE (1993) Increase in activity and level of pp60c-src in progressive stages of human colorectal cancer. J Clin Invest 91: 53–60
Wang NM, Yeh KT, Tsai CH, Chen SJ and Chang JG (2000) No evidence of correlation between mutation at codon 531 of src and the risk of colon cancer in Chinese. Cancer Lett 150: 201–204
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Laghi, L., Bianchi, P., Orbetegli, O. et al. Lack of mutation at codon 531 of SRC in advanced colorectal cancers from Italian patients. Br J Cancer 84, 196–198 (2001). https://doi.org/10.1054/bjoc.2000.1560
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DOI: https://doi.org/10.1054/bjoc.2000.1560
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