Figure 8 | Cancer Gene Therapy

Figure 8

From: In vitro activation of cancer patient–derived dendritic cells by tumor cells genetically modified to express CD154

Figure 8

Tumor-specific cytotoxic activity of CD8-positive splenocytes from mice vaccinated with MB49 expressing CD154. Mice were vaccinated by four weekly injections of MB49 transduced with Ad-CD154 and challenged by a single injection of parental MB49. Two to three weeks later CD8-positive splenocytes from vaccinated mice (A) and naïve control mice (B) were isolated and restimulated in vitro with irradiated MB49. Cytotoxicity of restimulated CTLs was tested against MB49, LLC1, and YAC1 in a standard cytotoxicity assay.

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