Figure 8
Concentration-dependent inhibition of WT and Y652A mutant hERG channels expressed in Xenopus oocytes. (A) Representative traces of WT and mutant hERG currents in the presence and absence of the indicated concentrations of hydroxyzine. The effects of the drug on WT and Y652A tail currents were recorded at −140 mV (instead of −60 mV as above) following a 4 s activating pulse. (B) The concentration-response curves were fitted with a logistic dose-response equation to yield IC50 values of 5.9±1.5 μmol/L (n=3) and 35.1±50.5 μmol/L (n=4) for WT and Y652A hERG channels, respectively.
