Abstract
Background:
Novel treatment strategies in Ewing sarcoma include targeted cellular therapies. Preclinical in vivo models are needed that reflect their activity against systemic (micro)metastatic disease.
Methods:
Whole-body magnetic resonance imaging (WB-MRI) was used to monitor the engraftment and dissemination of human Ewing sarcoma xenografts in mice. In this model, we evaluated the therapeutic efficacy of T cells redirected against the Ewing sarcoma-associated antigen GD2 by chimeric receptor engineering.
Results:
Of 18 mice receiving intravenous injections of VH-64 Ewing sarcoma cells, all developed disseminated tumour growth detectable by WB-MRI. All mice had lung tumours, and the majority had additional manifestations in the bone, soft tissues, and/or kidney. Sequential scans revealed in vivo growth of tumours. Diffusion-weighted whole-body imaging with background signal suppression effectively visualised Ewing sarcoma growth in extrapulmonary sites. Animals receiving GD2-targeted T-cell therapy had lower numbers of pulmonary tumours than controls, and the median volume of soft tissue tumours at first detection was lower, with a tumour growth delay over time.
Conclusion:
Magnetic resonance imaging reliably visualises disseminated Ewing sarcoma growth in mice. GD2-retargeted T cells can noticeably delay tumour growth and reduce pulmonary Ewing sarcoma manifestations in this aggressive disease model.
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Main
Ewing sarcoma is a cancer of bone and soft tissue that is characterised by a molecular rearrangement of the EWS gene on chromosome 22 with an ets-type gene (Delattre et al, 1992). Survival of patients with localised disease has improved substantially by modern multimodal treatment regimens (Paulussen et al, 1998), but primary multifocal disease or disseminated relapse often remains incurable (Ladenstein et al, 2010). Novel treatment strategies aim to eliminate residual microscopic disease remaining after standard therapy and include molecularly targeted drugs and antibodies (Erkizan et al, 2009; Juergens et al, 2011) as well as immunological therapies (Mackall et al, 2008; Kailayangiri et al, 2012). One approach is based on the genetic engineering of T cells with recombinant chimeric antigen receptors (CARs) directed against the ganglioside antigen GD2 that is overexpressed on many Ewing sarcomas (Kailayangiri et al, 2012). GD2-specific CARs consist of the antigen-binding domain of a GD2-specific antibody linked to T-cell receptor-signalling domains and mediate efficient cytolytic T-cell interaction with GD2-expressing tumour cells both in vitro and in vivo (Rossig et al, 2001; Pule et al, 2008).
In vivo preclinical data are often generated in localised tumour models, although the capacity of novel therapies to prevent the outgrowth of subcutaneous tumours in mice inadequately reflects their activity against systemic (micro)metastatic disease. To study the biology of systemic disease and to develop novel therapies including translational validation, preclinical in vivo models are needed that recapitulate the biology of multifocal disease and tumour dissemination. In accordance with the known haematological spread pattern of Ewing sarcoma, intravenous transplantation of single-cell suspensions of Ewing sarcoma cells into highly immunodeficient mice was found to efficiently establish multifocal sarcomas in many of the animals (Vormoor et al, 2001). The use of systemic disease models as preclinical tools for monitoring the antitumour activity of novel therapies requires visualisation of tumour growth in the living animal. Here, we used whole-body (WB) imaging techniques, including both standard magnetic resonance imaging (MRI) and diffusion-weighted WB imaging with background signal suppression (DWIBS) to follow-up the engraftment and systemic spread of human Ewing sarcoma xenografts in mice and to address the therapeutic efficacy of adoptive T-cell transfer against disseminated tumour cells in vivo.
Materials and methods
Cell lines
The Ewing sarcoma cell line VH-64 was a gift from Frans van Valen at the Institute of Experimental Musculoskeletal Medicine of University of Muenster, Germany. It was originally established from a malignant pleural effusion of a patient with Ewing sarcoma of the metatarsal bone. The identity of the cell line throughout the experiments was confirmed by repeated short tandem repeat profiling. VH-64 cells were cultured in collagen-coated 25 cm2 tissue culture flasks in RPMI 1640 medium (Invitrogen, Darmstadt, Germany), supplemented with 10% heat-inactivated foetal calf serum (Thermo Fisher, Bonn, Germany) and 2 mM L-glutamine and maintained at 37 °C and 5% CO2. The retroviral packaging cell lines Phoenix-ampho and FLYRD 18 were provided by Gary P Nolan (Stanford University School of Medicine, Stanford, CA, USA) and E Vanin (Center for Cell and Gene Therapy, Houston, TX, USA), respectively.
Xenogeneic NOD/scid mouse model of Ewing sarcoma
Mouse experiments were approved by the animal care committee of the local government (Bezirksregierung Muenster, Az. 87-51.04.2010.A117). NOD/scid mice (8–10-week old; Charles River Laboratories, Sulzfeld, Germany) were irradiated with a single dose of 3.5 Gy from a linear accelerator 1 day before transplantation. We chose VH-64 cells for this study, as the engraftment, dissemination, and in vivo proliferation of this cell line is highly representative of Ewing sarcoma xenografts (Vormoor et al, 2001). Single-cell suspensions of 2 × 106 tumour cells in 0.2 ml of medium were injected into the tail veins. All experimental manipulations were performed under sterile conditions in a laminar flow chamber. Animals developing clinical signs of disease (weight loss, shaggy fur, bent spine) were killed.
Whole-body MRI of mice
High resolution MR data sets were acquired on a clinical 3 Tesla WB MR system (Achieva, Philips Medical Systems, Best, the Netherlands) equipped with standard gradient coils (gradient strength 40 mT m−1, slew rate 200 mT m−1 ms−1). A dedicated small animal solenoid receiver coil (Philips Research, Hamburg, Germany) with an inner diameter of 40 mm and an integrated heating system to regulate the body temperature of the animals was used for signal reception. The MRI protocol consisted of the following sequences: T2 TSE sagittal (TR 1732 ms, TE 60 ms, FOV 70 (tail to head) × 30 (anterior-posterior) mm, reconstruction matrix 560 × 240, number of signal averages 4, slice thickness 1 mm, number of slices 27, scan time 3 : 34.8 min), T2 TSE axial (TR 2854 ms, TE 60 ms, FOV 32 (right to left) × 32 (anterior-posterior) mm, reconstruction matrix 288 × 288, NSA 4, slice thickness 1.5 mm, number of slices 45, scan time 05 : 53.9 min), STIR TSE sagittal (TR/TI 2445/200 ms, TE 68 ms, FOV 70 (tail to head) × 29 (anterior-posterior) mm, reconstruction matrix 512 × 214, NSA 14, slice thickness 1 mm, number of slices 25, scan time 07 : 59.3 min), DWIBS axial (TR/TI 16470/240 ms, TE 87 ms, FOV 50 (tail to head) × 50 (anterior-posterior) mm, reconstruction matrix 256 × 256, multi-shot EPI, EPI factor 9, number of b-factors 2 (0;1000), NSA 1, slice thickness 0.8 mm, gap 0.2 mm, number of slices 87, scan time 08 : 30.6 min; Takahara et al, 2004). Zero-filling was used to calculate the reconstruction matrix. Apparent diffusion coefficient maps were calculated from the native diffusion images with the built-in software tools of the MRI scanner. All sequences were acquired with the same geometry to assure comparability of the various sequences. First, MRIs were performed between 20 and 24 days after tumour cell transplantation independent of the development of disease symptoms. Up to four follow-up MRIs were performed to monitor tumour growth. Time points (TPs) were designated as follows: TP0, tumour cell transplantation; TP1, initial scan, median 20 days (range 20–24) after TP0; TP2, median 30 days (range 27–31) after initial scan; TP3, median 34 days (range 34–39) after initial scan; TP4, 41 days after initial scan. During examination, the mice were anaesthetised with isoflurane. Precautions to reduce the stress associated with general anaesthesia and imaging included maintenance and monitoring of the body temperature, transport to the imaging facility in suitable transport boxes, reduction of total imaging time to 1 h, and limitation to one imaging session per week. No contrast agent was given, and images were generated without respiratory gating. Two experienced radiologists independently evaluated all acquired images. Tumours were measured on the slice with their largest extent in T2 sagittal images in two directions (x, y), with an intraclass correlation of 0.958 between both investigators. Tumour volume estimation was done by manually tracing the tumours on each slice image, and then adding all the voxel volumes within the boundaries of the region of interest. Total volumes of individual tumours were calculated using Amira Software, Visage Imaging, Berlin, Germany.
Histopathology and immunohistochemistry
To confirm the presence of Ewing sarcoma xenografts, lungs, kidneys, and macroscopically detected bone and soft tissue tumours were examined by light microscopic examination and by immunohistochemistry. Tissues were fixed in 4% buffered formalin, processed and embedded in paraffin. Four-to five-micrometer sections were prepared and stained with haematoxylin and eosin or with the anti-human CD99 monoclonal antibody TÜ12 (BD Pharmingen, Heidelberg, Germany).
Production of recombinant retrovirus, transduction and expansion of T cells
Generation of the GD2-specific CAR 14.G2a-CD28ζ has been described in detail in previous publications from our group (Rossig et al, 2001; Altvater et al, 2006; Kailayangiri et al, 2012). The receptor contains the single-chain antibody domain of the monoclonal anti-GD2 antibody 14.G2a (Mujoo et al, 1989), the transmembrane domain of CD8α, and the intracellular domains of CD28 and TCR ζ. Retroviral supernatant was generated and used to transduce in vitro OKT-3/anti-CD28 antibody-preactivated human T cells, as previously described (Kailayangiri et al, 2012). To superexpand the gene-modified T cells, 2 × 107 cells were transferred to gas-permeable culture devices with 500 ml capacity (Wilson Wolf Manufacturing, New Brighton, MN, USA) in 400 ml of medium containing IL-2 (50 U ml−1) on day 5 after the prestimulation.
T-cell therapy
Following transplantation of 2 × 106 VH-64 tumour cells into the tail veins of NOD/scid mice, experimental cohorts of mice were given intravenous injections of 1 × 107 14.G2a-CD28ζ-transduced human T cells, whereas control cohorts received non-transduced T cells. In a first cohort of 10 mice (5 treated and 5 control mice), T-cell transfer was given on days 4, 10, 15, and 22 after tumour inoculation. A subsequent cohort of another eight mice (four treated and four control mice) received a total of six T-cell therapies on days 1, 3, 6, 9, 12, and 15. Intraperitoneal injections of human recombinant IL-2 were given twice weekly to all mice to sustain T-cell survival within the non-human environment. Mice receiving four and six injections were analysed together. Tumour development was monitored using WB-MRI as described above.
Statistical analysis
Statistical analyses were performed using IBM SPSS Statistics 21 for Windows (IBM Corporation, Somers, NY, USA) and SAS software, Version 9.3 of the SAS System for Windows (SAS Institute Inc., Cary, NC, USA). P-values were interpreted in Fisher’s sense, representing the metric weight of evidence against the respective null hypothesis of no effect, and were considered noticeable in case P⩽0.05 and highly noticeable in case P⩽0.01. The intraclass correlations coefficient (ICC) was calculated as a measure of inter-rater agreement. Continuous data are reported as median, minimum, and maximum. Categorical variables are described as absolute and relative frequencies. Relationships between categorical variables were compared via contingency tables, χ2-, and Fisher’s exact test, where appropriate. Exact χ2-goodness-of-fit test was applied to compare the allocation of tumour numbers between cohorts. Pairwise group comparison for tumour numbers and tumour volumes were performed using exact non-parametric Mann–Whitney U-tests. Wilcoxon-signed rank tests were applied to detect the statistically noticeable changes between two TPs. Kaplan–Meier analyses and log-rank tests were performed to compare event-free and overall survival between treated and control cohorts. Multivariable, mixed model analyses were conducted to analyse the impact of potentially explanatory variables on target parameters.
Results
Whole-body MRI visualises disseminated tumour growth after intravenous injection of xenogeneic Ewing sarcoma cells in mice
High quality images in mice were obtained using a clinical 3 Tesla MRI scanner without respiratory triggering and without the need for contrast-enhancing agents. Among the first cohort of mice (n=18) receiving intravenous injections of 2 × 106 VH-64 cells, all 18 mice developed disseminated tumours detectable by WB-MRI within 20–31 days (median of 27 days). All engrafted mice had lung tumours (100%). Sixteen mice had additional tumour manifestations in extrapulmonary sites: 55.6% of mice (10 of 18) had bone or bone marrow tumours in the lower extremities, pelvis and/or vertebral column, and 27.8% (5 of 18) had soft tissue tumours (Figure 1A). Moreover, 72.2% (13 of 18) of mice had tumours in the kidney, and three of these also had tumours within the suprarenal gland. One of five female mice and none of 13 male mice had an abdominal tumour, potentially representing an ovarial manifestation. The highest numbers of tumours were found in the lungs, with a median of 19.5 (range 1–60) per mouse (Figure 1B). Interobserver agreement was consistent with an overall ICC of 0.915 for tumour numbers and 0.967 when excluding lung tumours. The median volume of tumours at first detection varied with the tumour site (Figure 1C). Soft tissue and kidney tumours were detectable at relatively small median sizes of 8.94 mm3 (range 2.53–25.05 mm3) and 8.15 mm3 (1.24–40.3 mm3), respectively, whereas bone tumours were relatively large upon first detection (median 33.11 mm3, range 13.49–117.9 mm3). Lung tumours were detectable at very small volumes of <1 mm3. Dissection and histological analysis confirmed the presence of CD99+ small, blue, round cell tumours in bones, lungs, and kidneys in all examined cases (Figure 1D).
Sequential WB-MRI allows to monitor tumour growth in vivo
Sequential WB-MRI scans were performed until the mice had to be killed. T2 sagittal WB sequences revealed in vivo growth of tumours at all sites (Figure 2A), with heterogeneous kinetics. Soft tissue tumours increased in sizes at relatively slow rates, potentially owing to the early detection of very small tumours. As reliable volumetry of individual lung tumours is complicated by their diffuse infiltrative character, pulmonary involvement was monitored by combining volumes of all individual lung tumours for each lung. Moreover, lung tumours were quantified by numbers in axial images (Figure 2B). A continuous increase of the numbers of lung tumours both per lung and per mouse was found in follow-up scans (Figure 2B and C).
Diffusion-weighted WB imaging with background signal suppression allows for functional detection of Ewing sarcoma manifestations and growth in mice
Whole-body diffusion-weighted MRI with background body signal suppression is a functional imaging technique. Tumours are visualised based on the restricted diffusion of the malignant tissue architecture (Takahara et al, 2004). In our cohort of 18 mice, effective background signal suppression was obtained, except for fluids in the bladder, renal pelvis, and intestinal tract. Diffusion-weighted WB imaging with background signal suppression effectively visualised disseminated Ewing sarcoma growth in bones, retroperitoneal organs, and soft tissues, but not in lungs where susceptibility artefacts and respiratory motions cause signal loss (Wang et al, 2012). Sequential DWIBS demonstrated increases of tumour volumes over time (Figure 3A). Four tumours (three bone tumours, one kidney tumour) were visualised by DWIBS before they appeared in MR T2 sequences. To determine the sensitivity and specificity of DWIBS, standard T2-weighted sagittal MRI sequences were defined as standard. Interobserver agreement on the presence of individual tumours by DWIBS was 92%. Limited specificity of DWIBS was found for renal manifestations (Figure 3B), explained by signal artifacts in the vicinity of liquid-filled spaces. Tumours of the suprarenal gland were not detected by DWIBS, potentially owing to their close proximity to the diaphragm (Kwee et al, 2008). Moreover, DWIBS failed to detect the pelvic bone lesions detected on T2 MRI sequences in 3 of 13 examinations. Tumours undetectable by DWIBS were smaller than those reliably detected with both techniques (Figure 3C). All in all, these results demonstrate feasibility of detection of disseminated Ewing sarcoma growth in a small animal model using DWIBS sequences.
In vivo antitumour activity of GD2-retargeted T cells against established tumour xenografts
To demonstrate the therapeutic efficacy of adoptive T-cell transfer against disseminated tumour cells, further cohorts of mice were intravenously injected with 2 × 106 VH-64 cells/mouse, followed by four (n=5) to six (n=4) transfusions of 1 × 107 14.G2a-CD28ζ gene-modified human GD2-specific T cells (group A, n=9). Control mice (group B, n=9) received analogous injections of non-transduced T cells. Analytic end points were the number of mice developing tumour manifestations and the numbers and volumes of tumours at the individual sites. Two control mice failed to develop Ewing sarcomas. All mice with lung tumours also had extrapulmonary tumour manifestations. Abdominal tumours that in female mice potentially represent ovarial manifestations were found in 2 of 6 male and in 10 of 12 female mice. As these tumours could not be assigned to specific organs, they were excluded from the analysis.
The numbers of mice developing tumours and the numbers of tumours at extrapulmonary localisation sites were not noticeably different between treated and untreated mice (Figure 4A and B). Mice receiving gene-modified T-cell therapy had lower numbers of pulmonary tumours than control mice (Figure 4B). Soft tissue tumours at first detection had lower volumes in the treatment group (Figure 4C). Differences at further sites did not reach statistical significance. All tumour-engrafted mice in both groups had progressive disease over time (Figure 4D). For comparisons of tumour growth between the two groups, multivariable analysis according to a generalised linear-mixed model was performed to account for the complexity of the experimental system and to include the effects of time and localisation. For all localisations and TPs combined, the estimated tumour volume was reduced by 7.95 mm3 in the treatment group, but the difference was not statistically noticeable (P=0.0965; Table 1). As tumour localisation itself had a noticeable influence on tumour volume (P<0.0001), we next compared tumour growth at individual localisations. At non-pulmonary sites, the maximum sizes of tumours over the entire follow-up were not noticeably different between mice receiving T-cell therapy and non-treated control mice, with the exception of reduced sizes of femoral and tibial bone tumours at the TP 4 (P=0.056; n=7 (A), n=2 (B)). Noticeable differences between groups were identified for lung tumours (Figure 4E): Mice treated with GD2-redirected T cells had a growth delay of lung tumours, with both lower numbers and smaller volumes (Figure 4B and E). No overall survival (as defined in the figure legend) advantage was found for mice receiving T-cell therapy using Kaplan–Meier analysis (Figure 4F). Thus, GD2-retargeted T cells cannot completely prevent disseminated tumour growth in this systemic disease model, but are active to delay and reduce pulmonary disease manifestations.
Discussion
Paediatric bone and soft tissue sarcomas tend to spread to and recur in lungs as well as in the bone and bone marrow. Multifocal disease is the hardest challenge to further improving cure rates. For the development of novel therapies and their clinical translation, in vivo preclinical models are needed that adequately mimic the dissemination pattern of these diseases and allow for non-invasive monitoring of tumour growth and response to treatment.
Here, we have established a small animal imaging model of systemic Ewing sarcoma growth using MRI techniques. Compared with the more broadly applied bioluminescence small animal imaging techniques, MRI directly reflects high resolution sarcoma staging in human patients and thus facilitates clinical translation of our findings.
Intravenous injection of human Ewing sarcoma cells into immunodeficient mice reliably established the disease in the majority of mice within <5 weeks, confirming previous experience (Vormoor et al, 2001). Compared with the routine histology of specific organs and macroscopically affected tissues in deceased mice, sequential whole-body imaging allows more comprehensive detection of various tumour manifestations. Specifically, small tumours were detected at sites not included into routine examination in the previous study (Vormoor et al, 2001). Importantly, the non-invasive nature of MRI further allows for follow-up investigations of tumour growth, both during the natural course of the disease and upon therapeutic intervention. Indeed, MRI provided a highly reproducible representation of clinical Ewing sarcoma development in mice and revealed systemic treatment efficacy of GD2 CAR gene-modified T cells. Our model is easy to apply and has translational potential within a wider scale for novel therapeutic developments against this malignant disease.
Besides their use in small animal models, non-invasive whole-body imaging tools are clinically needed for accurate tumour staging and assessment of treatment response. MRI is one of the most important diagnostic tools in the bone and soft tissue tumours where it provides excellent anatomical information (Lang et al, 1998a, 1998b). Whole-body MRI is currently evaluated for the initial staging of patients with malignant solid tumours including Ewing sarcomas (Haubold-Reuter et al, 1993; Walker et al, 2000; Lauenstein et al, 2002). Its specific value lies within the early detection of bone and bone marrow disease (Eustace et al, 1997; Steinborn et al, 1999; Altehoefer et al, 2001; Lauenstein et al, 2002; Hargaden et al, 2003; Ghanem et al, 2006). MRI further allows for volume-based assessment of response to cancer therapy. A limitation is that it does not assess the metabolic activity of remaining tumour tissue. Diffusion-weighted MRI was introduced as a non-invasive technique providing functional information for both staging and response monitoring of solid tumours (Roth et al, 2004; Thoeny et al, 2005; Moffat et al, 2006; Schubert et al, 2006; Vandecaveye et al, 2009; Wang et al, 2009; Oka et al, 2010). In first preclinical studies in Ewing sarcomas, diffusion-weighted imaging effectively predicted early response of single subcutaneous tumours to chemotherapy in mice before the onset of morphological changes (Reichardt et al, 2009), and in a mouse model of ovarian cancer, DWIBS had the potential to detect peritoneal tumour dissemination (Lee et al, 2013). In pilot clinical studies, WB-MRI with DWIBS was found useful for detecting metastatic tumours especially in the bone (Nakanishi et al, 2007; Manenti et al, 2012; Sommer et al, 2012). Here, to the best of our knowledge, we are the first to demonstrate the feasibility of DWIBS for the staging of multifocal Ewing sarcoma in small animals. With the known limitation of DWIBS not detecting pulmonary metastases, the technique provided a rapid overview of extrapulmonary disease manifestations. The future value of the DWIBS technique in sarcoma staging may lie within providing complementary non-invasive functional tissue information for extrapulmonary sites of the disease, while avoiding exposure to ionising radiation.
Among novel experimental cancer therapies, adoptive transfer of T cells expressing recombinant antigen-specific CARs holds high promise. In patients with lymphoid malignancies, CAR-reengineered T cells mediated unequivocal clinical responses even against bulky tumours, functionally persisted in vivo, and established immunological memory (Kalos et al, 2011; Louis et al, 2011; Porter et al, 2011). However, results from individual clinical studies are variable (Brentjens et al, 2011; Kalos et al, 2011; Porter et al, 2011; Savoldo et al, 2011; Kochenderfer et al, 2012), and the reasons for the compelling activity of this therapy in some clinical studies but not in others are unclear. Besides the design of the individual CARs and the nature of their target antigen, host factors are thought to contribute to determining CAR treatment responses (Dudley et al, 2002). Although in our study, GD2 CAR gene-modified T cells were not effective to prevent the disease, they had noticeable antitumour activity especially against pulmonary dissemination. Thus, our findings support further development and translational application of cellular therapies in Ewing sarcoma. A potential reason for the limited antitumour activity of the reengineered T cells is the high number of disseminated tumour cells present in the mouse organism at the time of T-cell transfer. Lower numbers of tumour cells reflecting minimal residual disease are more probably to be eradicated by this and other experimental interventions, but are also less reliable to engraft the disease in all mice. High-sensitivity imaging, as established here, may allow to detect tumour manifestations mediated by smaller numbers of tumour cells and mimic minimal disseminated disease more accurately than current models. To more adequately reflect the spectrum of local progression and organ and tissue dissemination of the disease, future experiments will include additional cell lines and low-passage cultures.
The challenge now is to maximise the activity of the reengineered T cells, for example, by manipulation of costimulatory domains and optimal CAR design. Transfer of tumour antigen-specific T cells in the setting of advanced disease may be inadequate to control the disease. This is also supported by recently published in vivo experiments using T cells with native receptor specificity for EWS-FLI-1, which despite clear antitumour activity could not halt disease progression (Evans et al, 2012). Thus, combined approaches with cytotoxic therapies may be more adequate to exploit the benefit of T-cell therapies. Moreover, recent insights into the components of the inflammatory microenvironment that contributes to local progression in Ewing sarcoma suggest that combinations with chemokine targeting may improve the efficacy of T-cell strategies (Berghuis et al, 2012). Although xenograft models carry limitations for immunological treatment manoeuvers as they do not completely represent the immunological in vivo complexity of interactions between host, tumour, and treatment, a comparative assessment of the antitumour activity of various constructs in our mouse model may help to choose the most promising constructs for clinical translation.
Change history
06 August 2013
This paper was modified 12 months after initial publication to switch to Creative Commons licence terms, as noted at publication
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Acknowledgements
This work was supported by Grant #109566 from Deutsche Krebshilfe (to CR) and a grant from the University of Muenster Faculty of Medicine ‘Innovative Medizinische Forschung (IMF)’ program (to BA and CR).
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HK is an employee of Philips Healthcare, the manufacturer of the MRI equipment used in this work. The remaining authors declare no conflicts of interest.
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Liebsch, L., Kailayangiri, S., Beck, L. et al. Ewing sarcoma dissemination and response to T-cell therapy in mice assessed by whole-body magnetic resonance imaging. Br J Cancer 109, 658–666 (2013). https://doi.org/10.1038/bjc.2013.356
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DOI: https://doi.org/10.1038/bjc.2013.356
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