Figure 3

CTLA-4 and CD39 are co-expressed on the majority of intratumoral FOXP3⁺ Treg. (A) CTLA-4, TIM-3 and CD39 expression on intratumoral FOXP3⁺ Treg was analysed by flow cytometry. FOXP3⁺ and FOXP3⁻ cells were, respectively, evaluated for their frequency of CTLA-4⁺, CD39⁺ and TIM-3⁺ cells. The numbers represent fraction of CTLA-4⁺, TIM-3⁺ and CD39⁺ cells in FOXP3⁺ or FOXP3⁻ CD4⁺ T cells. (B) Percentages of double-positive CD39⁺CTLA-4⁺ or CD39⁺TIM-3⁺ cells in FOXP3⁺CD4⁺ T cells (open squares and circles) are compared with those in FOXP3⁻CD4⁺ T cells (filled squares and circles, n=9). Then, the percentage of double-positive CD39⁺CTLA-4⁺ cells was compared with that of CD39⁺TIM-3⁺ cells in FOXP3⁺ Treg.