Figure 6

Maml1^−/− mutant mice present foliation defects in developing cerebellum. (a) Midsagittal sections of E18.5 and E19.5 Maml1^−/− and control cerebella stained with H&E. The four principal fissures (denoted by asterisks) as well as two additional fissures (indicated by the arrows) are shown in the figure. Abbreviations: prc, precentral; pc, preculminate; pr, primary; pp, prepyramidal; sec, secondary; pl, posterolateral fissures; ABL, anterobasal; ADL, anterodorsal; CEL, central; POS, posterior; INL, inferior lobes. Images of each panel were taken at the same magnification. Scale bar: 250 μm. (b) Immunohistochemical staining for PCNA in midsagittal cerebellar sections from Maml1^+/+ and Maml1^−/− mice at E19.5 (left panels). Scale bar: 250 μm. High magnification images of PCNA staining in the EGL, corresponding to posterolateral (A, C) and secondary (B, D) fissures (right panels). Scale bar: 50 μm (c) Graph shows the number of PCNA-positive cells, analyzed as fold of reduction in comparison with Maml1^+/+ control mice. Five sections/mouse n=3 mice per group were analyzed. ****P≤0.0001 (d) Quantitative analyses of cerebellar morphology. Values of perimeter (upper) and area (lower) of midsagittal cerebella sections from three mice of each genotype are represented in the graphs (left panels). Littermates are indicated with the symbol of the same color. The histograms (right panels) show Maml1^−/− cerebellum perimeter (upper) and area (lower), represented as fold of reduction compared with control. *P≤0.05