Abstract
Helminth infection leads to the local proliferation and accumulation of macrophages in tissues. However, the function of macrophages during helminth infection remains unclear. SH2-containing inositol 5′-phosphatase 1 (Ship1, Inpp5d) is a lipid phosphatase that has been shown to play a critical role in macrophage function. Here, we identify a critical role for Ship1 in the negative regulation of interleukin (IL)-12/23p40 production by macrophages during infection with the intestinal helminth parasite Trichuris muris. Mice with myeloid cell-specific deletion of Ship1 (Ship1ΔLysM mice) develop a non-protective T-helper type 1 cell response and fail to expel parasites. Ship1-deficient macrophages produce heightened levels of IL-12/23p40 in vitro and in vivo and antibody blockade of IL-12/23p40 renders Ship1ΔLysM mice resistant to Trichuris infection. Our results identify a critical role for the negative regulation of IL-12/23p40 production by macrophages in the development of a protective TH2 cell response.
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Introduction
Protective immunity against helminth infection is associated with the development of a type 2 immune response, characterized by activation and expansion of CD4+ T-helper type 2 (TH2) cells that produce cytokines such as interleukin (IL)-4 and IL-13, induction of IgG1- and IgE-secreting plasma cells, as well as increased numbers of macrophages, eosinophils, mast cells, and basophils.1 Elevated levels of IL-4 and IL-13 result in increased intestinal permeability and enhanced mucus and protein secretion by goblet cells, and increased smooth-muscle cell contractility.2, 3, 4, 5, 6 In contrast, susceptibility to helminth infection is associated with a non-protective T-helper type 1 (TH1) response that results in high levels of IL-12, IL-18, and interferon (IFN)-γ that permits chronic infection.7, 8 As dysregulated TH2 cell responses are associated with a variety of inflammatory diseases including asthma and allergies, a better understanding of the cellular and molecular mechanisms that control TH2 cell development would be likely to reveal novel targets for therapeutic intervention in treating these diseases.
Macrophages are a highly plastic and functionally diverse subset of cells that play a key role in virtually all aspects of immune responses.9 Macrophages act as initiators of immune responses by presenting antigen to T cells, regulators of immunity by producing immunoregulatory cytokines such as IL-10 or TGFβ, and immune effectors through their phagocytic uptake and killing of bacterial, fungal, and protozoal pathogens by nitric oxide and reactive oxygen species-mediated processes.10, 11 However, the detailed molecular mechanisms that control the differentiation and function of various macrophage subsets, particularly during TH1 and TH2 responses, remain unclear.
The role of macrophages during helminth infection is controversial. Macrophage numbers increase significantly in the tissues following infection with a variety of helminth parasites,12, 13, 14, 15 and recent data in the peritoneal cavity suggest that this may be due to local IL-4-dependent proliferation rather than recruitment of precursors from the blood.16 Despite this expansion, it has proven difficult to show a clear functional link between their presence and resistance to helminth infection. Macrophages associated with anti-helminth TH2 cell responses adopt an alternative differentiation state, which has been termed M2.17, 18 The M2 macrophage phenotype is characterized by the expression of a set of genes including arginase 1 (Arg1).19 Increased Arg1 expression is associated with suppression of adaptive immune responses by depleting local concentrations of arginine, an essential amino acid.20 M2 polarization is mediated by IL-4 or IL-13, which both signal through the IL-4Rα chain, and accordingly IL-4Rα-deficient macrophages fail to acquire an M2 phenotype. Interestingly, mice with a macrophage-specific deletion of IL-4Rα develop normal protective TH2 cell-dependent immune responses following infection with the parasitic helminths Nippostrongylus brasiliensis21 or Trichinella spiralis.22 In addition, mice treated with Arg1 inhibitors or mice deficient in Arg1 are not impaired in their ability to clear many helminth parasite infections.23, 24 Furthermore, depletion of macrophages in resistant C57BL/6 mice during infection with the intestinal helminth parasite Trichuris muris25 has no effect on the development of protective responses and parasite expulsion.26 In summary, while macrophage differentiation and accumulation in infected tissues is a hallmark of helminth infections, the role of macrophages in the development of protective TH2 cell-dependent immunity is not clear.
SH2-containing inositol 5′-phosphatase 1 (Ship1, Inpp5d) is a lipid phosphatase that hydrolyses the 5′-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PI(3,4,5)P3) to produce PI(3,4)P2, thereby negatively regulating the phosphoinositide 3-kinase (PI3K) pathway. Ship1−/− mice have multiple immunological defects, including a myeloproliferative disorder, characterized by overproduction of granulocytes, mast cells,27 macrophages,28 and dendritic cells (DCs),29, 30 and elevated immunosuppressive myeloid-derived suppressor cells29 and regulatory T cells.31, 32 In addition, CD4+ T cells from Ship1−/− mice produce heightened levels of the TH2 cell-associated cytokines IL-4 and IL-13.33 Peritoneal and alveolar macrophages from naïve Ship1−/− mice display an M2 phenotype, expressing high levels of Arg1.34 In contrast, Ship1−/− bone marrow-derived macrophages (BMMacs) produce heightened levels of IL-6 and tumor necrosis factor-α in response to lipopolysaccharide (LPS) stimulation, fail to undergo LPS-induced tolerance, and Ship1−/− mice are highly sensitive to endotoxic shock.35
On the basis of these studies, we hypothesized that Ship1−/− mice would display heightened TH2 cell responses and increased resistance to helminth infection. Here we report that in contrast to our expectations, following infection with Trichuris Ship1−/− mice fail to develop a polarized protective TH2 cell response and are susceptible to infection. Furthermore, we have employed myeloid cell-specific Ship1-deficient mice (Ship1ΔLysM mice) to show that dysregulated macrophage function impacts the development of a protective anti-helminth response. We show that in normal macrophages, Ship1 is required to limit IL-12 production by macrophages and that this in turn is required for the development of a protective TH2 cell response. Taken together, our results reveal a surprising and previously unappreciated role for Ship1 in the development of protective immunity to helminth infection.
Results
Ship1–/– mice are susceptible to infection with Trichuris
It has previously been demonstrated that naïve Ship1–/– mice are biased towards type 2 immune responses, with increased levels of IgE, heightened production of type 2 cytokines by T cells and basophils, and the presence of M2 macrophages.33, 34, 35 On the basis of these previous studies, we hypothesized that Ship1−/− mice would display increased resistance to infection with the helminth parasite Trichuris. However, following infection with Trichuris, genetically resistant C57BL/6 mice display increased expression of Ship1 mRNA in the intestine (Figure 1a), suggesting that Ship1 expression is associated with immunity to infection. Consistent with this, Ship1−/− mice were more susceptible to Trichuris infection, failing to expel worms by day 21 (Figure 1b). Restimulation of mesenteric lymph node (mLN) cells isolated from infected Ship1−/− mice with Trichuris antigen resulted in decreased levels of IL-13 and heightened levels of IFN-γ when compared with Ship1+/+ mice (Figure 1c). Consistent with this skewed immune response, infected Ship1−/− mice also displayed lower levels of Trichuris-specific serum IgG1 and higher IgG2a than infected Ship1+/+ mice (Figure 1d). Although naïve Ship1+/+ and Ship1−/− mice were indistinguishable histologically (data not shown), infected Ship1−/− mice displayed increased inflammation, loss of crypt architecture, and lack of goblet cells compared with control mice (Figure 1e). While goblet cell numbers were equivalent between naïve Ship1+/+ and Ship1−/− mice (data not shown), following Trichuris infection there were significantly fewer goblet cells in the intestines of Ship1−/− mice (Figure 1f). Thus, Ship1−/− mice fail to generate a protective antigen-specific TH2 cell response during infection with Trichuris, developing instead a polarized non-protective TH1 cell response.
Ship1ΔLysM mice are susceptible to infection with Trichuris
We sought to test whether myeloid cell-specific Ship1 expression was required for resistance to intestinal helminth infection. We generated mice with a specific deletion of Ship1 in myeloid cells, including macrophages and neutrophils (Ship1ΔLysM mice) by crossing Ship1F/F mice with LysM-cre transgenic mice.36, 37, 38 Ship1F/F or Ship1ΔLysM mice were infected with Trichuris and analyzed on days 21 and 32 post-infection. Strikingly, Ship1ΔLysM mice failed to expel worms by day 32, while control Ship1F/F mice had cleared all parasites by day 21 (Figure 2a). Susceptibility to infection in Ship1ΔLysM mice was associated with increased levels of IFN-γ produced by mLN cells (Figure 2b) and Ifng gene expression in the intestine (Figure 2c). While we observed no difference in IL-4, IL-5, and IL-13 production by restimulated mLN cells between Ship1F/F or Ship1ΔLysM mice (Figure 2b and Supplementary Figure 1A), we did detect decreased levels of intestinal Il13 mRNA in Ship1ΔLysM mice (Figure 2c), but not Il4 or Il5 mRNA (Supplementary Figure 1B). Consistent with increased IFN-γ production, we also detected decreased levels of Trichuris-specific IgG1 and increased levels of IgG2a in the serum (Figure 2d). Furthermore, histological analyses revealed heightened inflammation in infected Ship1ΔLysM mice, with loss of crypt structure and elevated inflammatory cell infiltrates (Figure 2e), but a failure to induce goblet cell hyperplasia (Figure 2f) and expression and secretion of resistin-like molecule (RELM)β (Figure 2g). Thus, expression of Ship1 in myeloid cells is required for all key hallmarks of protective TH2 cell-dependent immunity to Trichuris. As macrophages have been shown to play a role in several helminth infections,12, 13, 14, 15, 16 we focused our studies on macrophages.
Ship1 controls expression of Arg1 in the peritoneal cavity but not the intestine
On the basis of previous studies using conventional Ship1−/− mice,34, 39 we hypothesized that macrophages from Ship1ΔLysM mice would express high levels of the M2 macrophage marker Arg1.19 Peritoneal macrophages isolated from Ship1ΔLysM mice displayed high levels of Arg1 as measured by mRNA expression (Figure 3a), protein production (Figure 3b), and enzymatic activity (Figure 3c). However, we observed no difference in the levels of Arg1 mRNA in the intestine of Ship1F/F or Ship1ΔLysM mice (Figure 3d). Expression of Arg1 is upregulated following infection with several helminth parasites, including Heligmosomoides polygyrus, N. brasiliensis, and Schistosoma mansoni.23, 40, 41 A recent study has demonstrated that during Trichuris infection, there is a mild increase in the frequency of Arg1-expressing cells in the intestine, but that Arg1 is not required for immunity to infection.24 While we observed an infection-induced increase in Arg1 gene expression in the intestine, we did not observe any differences between Trichuris-infected Ship1F/F and Ship1ΔLysM mice (Figure 3d). Consistent with these results, treatment of mice with the Arg1 inhibitor (S)-(2-boronoethyl)-L-cysteine (BEC) had no effect on the outcome of Trichuris infection in Ship1F/F or Ship1ΔLysM mice (data not shown). We also failed to observe any differences in the expression of another M2 cell marker, RELMα (Retnla) (data not shown). However, we did observe significantly increased expression of inducible nitric oxide synthase (Nos2), a marker for M1 macrophages, consistent with the heightened levels of IFN-γ and IL-12 observed in the intestines of Ship1ΔLysM mice (Figure 3e). Thus, although Ship1 controls Arg1 expression in peritoneal macrophages, it has no effect on Arg1 expression in the intestine. Furthermore, we conclude that the susceptibility of Ship1ΔLysM mice to Trichuris infection is not due to the presence of dysregulated M2 macrophages or expression of Arg1.
Antibody blockade of IFN-γ in Trichuris-infected Ship1ΔLysM mice promotes resistance
As we observed heightened levels of IFN-γ in infected Ship1ΔLysM mice (Figure 2b), we next asked whether antibody blockade of IFN-γ would render Ship1ΔLysM mice resistant to infection. Treatment with α-IFN-γ antibody reversed the susceptibility to Trichuris infection in Ship1ΔLysM mice, with a significant reduction in worm burden in antibody-treated mice (Figure 4a). Antibody treatment also resulted in increased IL-13 and decreased IFN-γ production by mLN cells (Figure 4b), as well as reduced levels of Trichuris-specific IgG2a in the serum of infected mice (Figure 4c). Increased resistance was associated with increased numbers of goblet cells and increased expression and secretion of RELMβ (Figure 4d and e). Thus, these results suggest that macrophage-specific expression of Ship1 is not required for resistance to Trichuris infection when non-protective TH1 cell responses are blocked.
Ship1−/− macrophages produce increased levels of IL-12p40 in vitro and in vivo
Consistent with the high levels of IFN-γ observed in Trichuris-infected Ship1ΔLysM mice, mLN cells isolated from infected Ship1ΔLysM mice cultured in either medium alone or in the presence of Trichuris antigen produced significantly increased levels of IL-12/23p40 (Figure 5a). We also observed heightened levels of Il12a and Il12b gene expression in the intestine of Ship1ΔLysM mice following Trichuris infection (Figure 5b). Finally, LPS stimulation of BMMacs derived from Ship1F/F or Ship1ΔLysM mice revealed increased levels of IL-12/23p40 production from Ship1-deficient macrophages (Figure 5c). Thus, Ship1 is intrinsically required to limit the production of IL-12/23p40 by macrophages.
Antibody blockade of IL-12p40 in Trichuris-infected Ship1ΔLysM mice promotes resistance
To test directly whether dysregulated production of IL-12 was responsible for the enhanced susceptibility of Ship1ΔLysM mice to Trichuris infection, we treated Ship1ΔLysM mice with a blocking antibody against IL-12/23p40 during Trichuris infection. Blockade of IL-12/23p40 in Ship1ΔLysM mice resulted in increased resistance to infection, characterized by reduced worm burden (Figure 6a), heightened IL-13, and decreased IFN-γ production by restimulated mLN cells (Figure 6b), and gene expression in the intestine (Figure 6c). In addition, elevated serum levels of Trichuris-specific IgG1 (Figure 6d), increased goblet cell hyperplasia (Figure 6e and f), and elevated expression and secretion of RELMβ into the intestinal lumen (Figure 6g) were all observed after anti-IL-12/23p40 treatment. Taken together, these results demonstrate that Ship1-dependent negative regulation of IL-12/23p40 production by macrophages is a key step required for the development of protective TH2 cell immunity to helminth infection.
Discussion
Here we demonstrate a role for macrophages in determining the adaptive immune response during helminth infection. In the absence of Ship1, macrophages produce increased levels of IL-12 and promote non-protective TH1 cell responses during infection with Trichuris. Blockade of IL-12/23p40 in Ship1ΔLysM mice leads to the restoration of a TH2 cell response and immunity to infection. Thus, although macrophages are not required for the development of anti-helminth immune responses, dysregulated macrophage functions can dramatically impact the outcome of infection. This study highlights a specific and critical role for macrophages during intestinal immune responses.
Conventional Ship1−/− mice display prominent macrophage skewing towards an M2 phenotype with alveolar and peritoneal macrophages expressing high levels of Arg1.34 While we also find that peritoneal macrophages from Ship1ΔLysM mice expressed high levels of Arg1, we were surprised to find that intestinal tissues from Ship1ΔLysM mice do not display heightened expression of Arg1. Consistent with these results, a recent study using conventional Ship1−/− mice showed only a modest 1.8-fold increase in the number of Arg1-positive macrophages in the large intestine in the steady state.39 Thus, it appears that Ship1 is differentially required for inhibition of M2 macrophage differentiation in a tissue-specific manner.
The role of macrophages during helminth infection has been a matter of controversy. Following infection with H. polygyrus, there is a 20-fold increase in Arg1-positive macrophages that are found surrounding tissue-embedded parasites and are critical for parasite expulsion.12, 41 In contrast, while macrophage numbers significantly increase in the intestine during Trichuris infection,42, 43 Arg1-positive macrophage numbers do not demonstrate a striking increase.24 Further, mice treated with an Arg1 inhibitor (BEC), macrophage-specific Arg1 knockout mice, or mice depleted of macrophages are all resistant to Trichuris infection, demonstrating that macrophages and/or Arg1 activity are not absolutely required for immunity.26 However, our results show that if macrophage responses are dysregulated (i.e., increased IL-12 production), immunity to Trichuris is abolished. Thus, there is a critical regulatory role for Ship1 in the development of type 2 immunity through the inhibition of type 1 responses. These data are reminiscent of previous work in which we show that the cytokine thymic stromal lymphopoietin does not directly promote TH2 cell responses, but is required to limit non-protective TH1 cell development by blocking IL-12 production.44 Thus, vaccines or therapeutic strategies aimed at enhancing TH2 cell responses should take the potential negative regulation of type 1 responses into consideration.
There are some instances where macrophages have been shown to play a critical role during helminth infection. Anthony et al.12 have demonstrated that macrophages can act as effector cells during infection with the helminth H. polygyrus, as depletion of macrophages using clodronate-loaded liposomes resulted in failure to kill tissue-embedded parasites. Furthermore, during infection with S. mansoni, abrogation of M2 macrophage function by macrophage-specific deletion of IL-4Rα or Arg1 results in heightened non-protective T-cell responses, severe liver fibrosis, and tissue damage, leading to increased mortality.21, 45 These results suggest that macrophages play a critical role in certain helminth infections primarily to prevent tissue damage and mortality.
The absence of Ship1 enzymatic activity results in increased levels of PI(3,4,5)P3 that could lead to increased activation of the PI3K pathway. There are many conflicting reports regarding whether the PI3K pathway has a positive or negative role in TLR-induced production of IL-12/23 and other proinflammatory cytokines.46, 47 Deletion of the PI3K regulatory subunit p85α resulted in increased IL-12 production by DCs.48 In contrast, recent studies specifically addressing the role of the PI3K p110α and p110β isoforms in LPS-induced IL-12 production suggest that the PI3K pathway positively regulates IL-12 production.49, 50 In addition, infection of Ship1−/− BMMacs with Francisella novicida resulted in increased expression of IL-12/23p40 associated with heightened levels of phosphorylated Akt, a marker for activation of the PI3K pathway.51 Thus, our results are consistent with these latter studies and suggest that Ship1 is a negative regulator of IL-12 production, possibly by regulating the levels of PI(3,4,5)P3.
In this study, we have used LysM-cre transgenic mice to delete Ship1 in myeloid cells, including macrophages. However, as LysM is also expressed in neutrophils, this cell type may also be playing a role in heightened IL-12 responses and increased susceptibility to Trichuris infection. Future studies using mice with more specific targeted Cre expression will identify whether Ship1 plays a role in macrophages, neutrophils, or both cell types.
In summary, our results identify a previously unappreciated role for macrophages in immunity to Trichuris. This study suggests that macrophages are not passive players in the development of protective type 2 responses, but can govern the outcome of an infection through modulation of the type of immune response. Future studies examining how Ship1 negatively regulates IL-12 expression will shed light on potential novel regulatory mechanisms that control immunity and inflammation.
Methods
Mice. LysM-Cre mice were obtained from The Jackson Laboratory (Bar Harbor, ME). The conditional Ship1 allele was generated as described previously.52 We observed no Cre toxicity as Cre+ Ship1F/+ mice were resistant to infection. Animals were maintained in a specific pathogen-free environment and tested negative for pathogens in routine screening. All experiments were carried out at the University of British Columbia following institutional and Canadian Council on Animal Care (CCAC) guidelines approved by the UBC Committee on Animal Care.
Parasites and infections. Isolation of Trichuris excretory–secretory antigen and eggs was carried out as described previously.53 Mice were infected on day 0 with 200–250 embryonated eggs, and parasite burdens were assessed microscopically on day 21 or 32 post-infection.
Analysis of Trichuris -induced immunity. Single-cell suspensions from mLN of naïve or Trichuris-infected mice were plated at 3–4 × 106 per ml in the medium or in the presence of antibodies against CD3 (145-2C11) and CD28 (37.51; 1 μg ml−1 each; eBioscience, San Diego, CA) or Trichuris antigen (50 μg ml−1) for 72 h. Cytokine production from cell-free supernatants was determined by standard sandwich enzyme-linked immunosorbent assay (ELISA) using commercially available antibodies (eBioscience). Trichuris-specific serum IgG1 and IgG2a levels were determined by ELISA on plates coated with Trichuris Ag (5 μg ml−1). Total protein was isolated from fecal samples, resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and immunoblotted using a rabbit anti-mouse RELMβ antibody (PeproTech, Rocky Hill, NJ).2 Cecal tissue samples were fixed in 4% paraformaldehyde and paraffin-embedded. Tissue sections were stained with periodic acid-Schiff (PAS) for visualization of goblet cells. Slides were analyzed on a Zeiss Axioplan2 microscope and images captured using a Qimaging Retiga EX CCD camera and the Openlab 4.0.4 software (Perkin-Elmer, Waltham, MA).
Quantitative RT-PCR. mRNA was isolated from tissue or cell samples using TRIzol according to the manufacturer's instructions Invitrogen (Grand Island, NY). Reverse transcription was used to generate cDNA and quantitative polymerase chain reaction was performed using SYBR green (Fermentas, Burlington, ON). The primers used for quantitative polymerase chain reaction analysis were the following: Actb forward, 5′-ACTAATGGCAACGAGCGGTTC-3′ and reverse, 5′-GGATGCCACAGGATTCCATACC-3′; Arg1 forward, 5′-CAGAAGAATGGAAGAGTCAG-3′ and reverse, 5′-CAGATATGCAGGGAGTCACC-3′; Ship1 forward, 5′-ACTCTGCGTGCTGTTCCGGA-3′ and reverse, 5′-CCTTCGGATGCCTGAACAGTG-3′; Nos2 forward, 5′-CGAAACGCTTCACTTCCAA-3′ and reverse, 5′-TGAGCCTATATTGCTGTGGCT-3′. All other primers were purchased from Qiagen (Germantown, MD). Reactions were carried out in an ABI 7900 real-time PCR machine (Applied Biosystems, Carlsbad, CA). Values are expressed relative to β-actin.
Bone marrow-derived macrophages. BMMacs were generated by culturing bone marrow cells (1 × 106 cells per ml) with recombinant macrophage colony-stimulating factor (10 ng ml−1; StemCell Technologies, Vancouver, BC) for 8 days. On day 8, macrophages were harvested and plated at 5 × 106 cells per ml and stimulated with LPS (50 ng ml−1; Sigma, St Louis, MO) for 24 h. Cell-free supernatants were collected and analyzed by ELISA.
In vivo antibody treatment. Monoclonal antibodies against IFN-γ (XMG-1.2) and IL-12/23p40 (C17.8) were purchased from Bio-X-Cell (West Lebanon, NH). Mice were treated intraperitoneally with 1 mg of antibody every 4 days between days 4 and 20 post-infection.
Statistics. Results represent mean±s.e.m. Statistical significance was determined by Student's t-test using Prism 4.0 (GraphPad Software, La Jolla, CA).
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Acknowledgements
We thank Dr Ken Harder for critical reading of the manuscript. This work was supported by the Canadian Institutes of Health Research (MOP-89773 and MOP-106623 to C.Z. and MOP-93580 and MOP-84545 to K.M.M.), Canada Foundation for Innovation (to C.Z.), the National Institutes of Health (RO1 HL72523, RO1 HL085580, RO1 HL107127-01 to W.G.K.), and the Paige Arnold Butterfly Run (to W.G.K.). F.A. is the recipient of a CIHR/Canadian Association of Gastroenterology/Crohn's and Colitis Foundation of Canada postdoctoral fellowship. W.G.K. is the Murphy Family Professor of Children's Oncology Research; K.M.M. is a Michael Smith Foundation for Health Research Senior Scholar; and C.Z. is a CIHR New Investigator and a MSFHR Career Investigator.
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Hadidi, S., Antignano, F., Hughes, M. et al. Myeloid cell-specific expression of Ship1 regulates IL-12 production and immunity to helminth infection. Mucosal Immunol 5, 535–543 (2012). https://doi.org/10.1038/mi.2012.29
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DOI: https://doi.org/10.1038/mi.2012.29