Extended Data Figure 5: p53−/− embryos do not exhibit characteristics of CHARGE syndrome.
From: Inappropriate p53 activation during development induces features of CHARGE syndrome
a, Whole-mount image of the external ear of an E15.5 p53−/− embryo (right) and a control embryo (left), showing normal ear pinna development. Original magnification, ×100. b, Whole-mount image of an E13.5 p53−/− embryo (right) and a control embryo (left), showing normal retinal development and no evidence of coloboma. Original magnification, ×100. c, Whole-mount image of an E15.5 p53−/−embryo (right) and a control embryo (left), showing normal lower jaw development. Original magnification, ×40. d, Top, Alizarin red (bone) and Alcian blue (cartilage) stained whole-mount E14.5 p53−/−embryo (right), showing normal long bone formation of the ulna (u), humerus (h), mandible (m), and femur (f) relative to littermate controls (left). Bottom, Quantification of the bone lengths shown as a percentage of E14.5 littermate controls (n = 3). Original magnification, ×6.3. e, Representative images of haematoxylin and eosin stained sagittal sections of E13.5 control hearts (left) and p53−/− hearts (right), showing all three cardiac cell types in both genotypes. en, endocardium; ep, epicardium; myo, myocardium (arrows). Original magnification, ×200. f, Analysis of haematoxylin and eosin stained transverse sections of E13.5 p53−/− and control hearts revealing normal outflow tract development. Top, The MPA and Ao are fully septated, and the MPA connects to the right ventricle (RV) in p53−/− hearts. Bottom, The Ao connects to the left ventricle (LV). The symbol Φ denotes that the ventricular outflow tract connects the LV and AO. AV, aortic valve; PV, pulmonary valve. Original magnification, ×100. g, Analysis of transverse sections of haematoxylin and eosin stained E13.5 p53−/− hearts (right) reveals normal atrioventricular cushions, which have undergone remodelling to form mature, elongated mitral valves (mv; arrowhead) and tricuspid valves (tv; arrow) similar to in control hearts (left). LA, left atrium; RA, right atrium. Original magnification, ×100. h, Haematoxylin and eosin stained sagittal section of kidney from p53−/− (right) and control (left) embryos, showing normal renal size and development. Original magnification, ×200. i, Haematoxylin and eosin stained transverse section of thymus in a p53−/− E13.5 embryo (right) reveals a similar thymus size to that in a littermate control (left). Original magnification, ×200.
