Extended Data Figure 7: Purified mEPF2 and Stomagen recombinant peptides and separation of bioactive mEPF2 by reverse-phase chromatography.
From: Competitive binding of antagonistic peptides fine-tunes stomatal patterning
a, SDS–PAGE gel of purified and refolded mEPF2–MYC–HIS and Stomagen recombinant peptides (asterisks). Left: molecular mass markers. b, HPLC chromatogram of purified, refolded mEPF2. Peaks 1 and 2 in UV chromatogram were collected and subjected to bioassays. c, Confocal image of cotyledon epidermis from wild-type seedling grown a solution with peak 1 for 5 days. No stoma is visible, indicating that peak 1 contains bioactive mEPF2. Scale bar, 20 μm. n = 19. d, Confocal image of cotyledon epidermis from wild-type seedling grown in a solution with peak 2 for 5 days, with normal stomatal differentiation, indicating that the peptide is not bioactive. Scale bar, 20 μm. n = 9.
