Extended Data Figure 4: Growth and ovule-targeting of prk mutant pollen tubes on the septum surface.

a–g, Entire images of growth and ovule-targeting of wild-type (a), prk6 (b), prk3 prk6 (c, d), prk3 prk6 prk8-2 (e, f), and prk1 prk3 prk6 (g) pollen tubes on the septum surface in wild-type pistils. Arrows indicate the tip of the longest pollen tube on the septum surface. Asterisks mark ovules that did not attract near pollen tubes. The regions shown in a, b and d are shown in Fig. 2e, f and g, respectively, as higher magnification images. Data are representative of 1–3 images for each genotype. Similar growth properties were observed in a total of 4 samples. Scale bar, 500 μm. Quantitative analysis is shown in Fig. 2d. No analysis was performed for the prk1 prk3 prk6 mutant because almost no pollen tube reached the ovule.