Extended Data Figure 4: Characterization of the Mfd–RNAP intermediate on negatively supercoiled 1 kb DNA in the absence or presence of UvrAB using the tethered-DNA assay.
From: Reconstruction of bacterial transcription-coupled repair at single-molecule resolution
DNA bears a C-less cassette. a, Nanomanipulation time-trace obtained in continuous-tracking mode in the presence of 25 pM RNAP holoenzyme, 100 nM Mfd, 500 nM GreB, 2 mM ATP, 200 μM UTP and 200 μM GTP. b, As in a but in the added presence of 50 pM UvrA and 250 nM UvrB. c, Lifetime distribution of the Mfd–RNAP intermediate in the absence of UvrA and UvrB has a mean lifetime of 258 ± 17 s (s.e.m., n = 33 events). d, Lifetime distribution of the Mfd–RNAP intermediate in the added presence of 50 pM UvrA and 250 nM UvrB has a mean lifetime of 167 ± 17 s (s.e.m., n = 32 events). Data sets for kinetics were obtained using the pulse-chase methodology.
