Extended Data Figure 1: Single-cell RNA-seq analysis of human oligodendroglioma samples.
From: Single-cell RNA-seq supports a developmental hierarchy in human oligodendroglioma
a, Experimental workflow. b, Clinical information of the main and validation patient cohorts analysed in this study. Asterisk indicates a borderline result of chromosome 1p loss based on clinical testing. c, ISH (top left) and FISH (all other panels) in a representative tumour (MGH36). All our cases retain ATRX protein expression by ISH (top left) and show loss of chromosomes arms 1p (bottom left) and 19q (top right) by FISH. In addition, tumour-specific CNVs identified by single-cell RNA-seq were confirmed by FISH (for example, loss of chromosome 4 in MGH36, bottom right panel). d, Distributions of the total number of sequenced paired-end reads per cell (grey) and of paired-end reads that were mapped to the transcriptome and used to quantify gene expression (black).
