Extended Data Figure 4: Responses of ganglion neurons in T1r3::SEMA3A mice.

a, Dose responses to sweet and bitter in control and T1r3::SEMA3A mice. The number of cells that respond at each stimulus concentration is shown, cells are classified by their response profile (that is, sweet-responding, bitter-responding or sweet-bitter responding). Note that the tuning profiles are maintained at all three sweet and bitter concentrations, including extremely high concentrations of bitter¹⁰ and sweet⁹. Control, n = 58 cells; T1r3::SEMA3A, n = 34 cells. b, Responses are similar for different tastants within a modality. Tuning properties of bitter- and sweet-responding neurons in the T1r3::SEMA3A animals to two structurally different bitter- and sweet-tasting chemicals are shown. Quinine (5 mM), cycloheximide (Cyx, 100 μM), AceK (30 mM) and sucrose (300 mM). Control, n = 178 bitter-responding cells, n = 227 sweet-responding cells (seven mice). T1r3::SEMA3A, n = 130 bitter-responding cells, n = 165 sweet-responding cells (five mice).