Extended Data Figure 8: NPL4 aggregation immobilizes the p97 binding partner and induces a global cellular HSR.
From: Alcohol-abuse drug disulfiram targets cancer via p97 segregase adaptor NPL4
a, Immobilization of selected proteins in Triton X-100-resistant pellet fractions of CuET-treated (1 μM, 3 h) U2OS cells. b, Immobilization of selected proteins in Triton X-100-resistant pellet fractions from U2OS cells expressing doxycycline-inducible NPL4(MUT)–GFP (48 h after induction). c, CuET dose-dependent immobilization of p97 in Triton X-100 pre-extracted MDA-MB-231 cells (3 h). Scale bar, 10μm. d, Immunohistochemical staining showing non-extractable p97 in MDA-MB-231 xenografts from mice treated with DSF or DSF and CuGlu, compared to vehicle. Scale bar, 50μm. e, HSR after CuET (8 h treatment) is shown by various HSR markers detected by western blotting of U2OS cell extracts. f, HSR markers in U2OS cells expressing doxycycline-inducible NPL4(MUT)–GFP (24 h after induction). a–f, Data are representative of two independent biological experiments.
