Extended Data Figure 7: p53 deficiency suppresses peripheral-blood cytopenias and ethanol-induced bone-marrow failure in Aldh2^−/−Fancd2^−/− mice.

a, Full blood count analysis of Aldh2^−/−Fancd2^−/−Trp53^−/− and control mice (8-to-12 weeks old, on a C57BL/6 × 129S4S6/Sv F1 background). A significant increase in the number of white blood cells, red blood cells, platelets and haematocrit was observed in Aldh2^−/−Fancd2^−/−Trp53^−/− mice compared to Aldh2^−/−Fancd2^−/− mice (P calculated by two-sided Mann–Whitney test; data shown as mean and s.e.m.; n = 17, 16, 21, 14, 18, 12, 18 and 12 mice, left to right). b, Aldh2^−/−Fancd2^−/−, Aldh2^−/−Fancd2^−/−Trp53^−/− and control mice were treated with ethanol in their drinking water for 10 days as described previously⁶. Full blood-count analyses were carried out after 10 days of ethanol treatment. c, Bone marrow cellularity after 10 days of ethanol treatment. In b, c, P calculated by two-sided Mann–Whitney test; data shown as mean and s.e.m.; n = 5, 6, 8, 6, 6, 4, 6 and 5 mice, left to right. d, Haematoxylin and eosin staining of bone-marrow sections 10 days after ethanol treatment (original magnification, ×100).