Supplementary Figure 4: Efficient silencing of sFLT1 mRNA in liver and kidney by hsiRNAsFLT1-2283 in pregnant mice. | Nature Biotechnology

Supplementary Figure 4: Efficient silencing of sFLT1 mRNA in liver and kidney by hsiRNAsFLT1-2283 in pregnant mice.

From: RNAi modulation of placental sFLT1 for the treatment of preeclampsia

Supplementary Figure 4

Pregnant CD1 mice were injected at day E14 and E15 with 2X20 mg/kg of hsiRNAsFLT1 -2283 (IV, tail vein) and sacrificed at day E19. a) sFLT1-i13 mRNA levels were measured using QuantiGene® assay (Affymetrix), normalized to housekeeping gene, and presented as % of PBS control (n=6 biologically independent animals, mean + s.d., ****P<0.0001, one-way ANOVA). b) Amount of guide strand accumulated in mouse tissues measured by PNA hybridization assay (n=6 biologically independent samples, boxes represent 25 to 75 percentiles and whiskers indicate minimal and maximal values, ****P<0.0001, one-way ANOVA).

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