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Isolation and Purification of Protein Granules from Escherichia coli Cells Overproducing Bovine Growth Hormone

Bio/Technology volume 3, pages 151–154 (1985)Cite this article

Abstract

High-level expression of bovine growth hormone in Escherichia coli results in the formation of distinct cytoplasmic granules that are visible with the phase-contrast microscope. These granules were examined by transmission and scanning electron microscopy. Intact granules were isolated from crude cell lysates by differential centrifugation and were further purified by a simple washing procedure that yields nearly homogeneous bovine growth hormone.

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Authors and Affiliations

  1. Division of Molecular and Cell Biology, Lilly Research Laboratories, Indianapolis, Indiana, 46285

    Ronald G. Schoner, Lee F. Ellis & Brigitte E. Schoner

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  1. Ronald G. Schoner
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  2. Lee F. Ellis
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  3. Brigitte E. Schoner
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Schoner, R., Ellis, L. & Schoner, B. Isolation and Purification of Protein Granules from Escherichia coli Cells Overproducing Bovine Growth Hormone. Nat Biotechnol 3, 151–154 (1985). https://doi.org/10.1038/nbt0285-151

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