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Group I Introns: New Molecular Mechanisms for mRNA Repair

Bio/Technology volume 13, pages 323–326 (1995)Cite this article

Using ribozymes to repair transcripts may yield new therapeutic approaches to genetic disease

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References

  1. Beebe, J.A. and Fierke, C.A. A kinetic mechanism for cleavage of precursor tRNAAsp catalyzed by the RNA component of Bacillus subtilis ribonuclease P. Biochemistry 33: 10294–10304. 1994.

    Article  CAS  Google Scholar 

  2. Young, B., Herschlag, D. and Cech, T.R. Mutations in a nonconserved sequence of the tetrahymena ribozyme increase activity and specificity. Cell 67: 1007–1019. 1991.

    Article  CAS  Google Scholar 

  3. Piccirilli, J.A., Vyle, J.S., Caruthers, M.H. and Cech, T.R. Metal ion catalysis by the tetrahymena rybozyme. Nature 361: 85–88 1993.

    Article  CAS  Google Scholar 

  4. Cech, T.R., Herschlag, D., Piccirilli, J.A. and Pyle, A.M. RNA catalysis by a group I ribozyme. J. Biol. Chem 267: 17479–17482. 1992.

    CAS  PubMed  Google Scholar 

  5. Strobel, S., Cech, T.R. Tertiary interactions with the internal guide sequence mediate docking of the P1 helix into the catalytic core of the tetrahymenea ribozyme. Biochemistry 32: 13593–13604. 1993.

    Article  CAS  Google Scholar 

  6. Moore, M.J. and Sharp, P.A. Site-specific modification of pre-mRNA: The 2′-hydroxyl groups at the splice sites. Science 256: 992. 1992.

    Article  CAS  Google Scholar 

  7. Strobel, S.A. and Cech, T.R. Minor groove recognition of the conserved GU pair at the tetrahymena ribozyme reaction site. Science 267: 675–679. 1995.

    Article  CAS  Google Scholar 

  8. Sullenger, B.A. and Cech, T.R. Ribozyme-mediated repair of defective mRNA by targeted trans-splicing. Nature 371, 619–622. 1994.

    Article  CAS  Google Scholar 

  9. Sullenger, B.A. unpublished research.

  10. Herschalg, D. and Cech, T.R. Catalysis of RNA cleavage by the tetrahymena thermophila ribozyme. Biochemistry 29: 10159–10171. 1990.

    Article  Google Scholar 

  11. Michel, F. and Westhof, E. Modelling of the three-dimensional architecture of group I catalytic introns based on comparative sequence analysis. J. Mol. Biol. 216: 585–610. 1990.

    Article  CAS  Google Scholar 

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Author notes

  1. Thomas Cech: This article was excerpted from Dr. Cech's Feodor Lynen Lecture, delivered jointly with Dr. Sidney Altman at the Miami Bio/Technology Winter Symposium, Fort Lauderdale, FL, in February.

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  1. distinguished professor of chemistry and biochemistry at the University of Colorado at Boulder, Howard Hughes Medical Institute, Boulder, CO, 80309-0215

    Thomas Cech

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  1. Thomas Cech
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Cech, T. Group I Introns: New Molecular Mechanisms for mRNA Repair. Nat Biotechnol 13, 323–326 (1995). https://doi.org/10.1038/nbt0495-323

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