Abstract
We introduced the potato proteinase inhibitor II (PINII) gene (pin2) into several Japonica rice varieties, and regenerated a large number of transgenic rice plants. Wound-inducible expression of the pin2 gene driven by its own promoter, together with the first intron of the rice actin 1 gene (actl), resulted in high-level accumulation of the PINII protein in the transgenic plants. The introduced pin2 gene was stably inherited in the second, third, and fourth generations, as shown by molecular analyses. Based on data from the molecular analyses, several homozygous transgenic lines were obtained. Bioassay for insect resistance with the fifth-generation transgenic rice plants showed that transgenic rice plants had increased resistance to a major rice insect pest, pink stem borer (Sesamia inferens). Thus, introduction of an insecticidal proteinase inhibitor gene into cereal plants can be used as a general strategy for control of insect pests.
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References
Heinrichs, E.A., Medrano, F.G., and Rapusas, H.R. 1985. Evaluation for Insect Resistance in Rice. International Rice Research Institute, Los Baños, Philippines.
Ling, K.C. 1972. Rice Virus Diseases. International Rice Research Institute, Los Baños Philippines.
Dulmage, H.T. 1981. Insecticidal activity of isolates of Bacillus thuringiensis and their potential for pest control, pp. 193–222 in Microbial Control of Pests and Plant Diseases 1970-1980. Burges, H. D. (ed.). Academic Press, New York.
Fischhoff, D.A., Bowdish, K.S., Perlak, F.J., Marrone, P.G., McCormick, S.H., Niedermeyer, J.G., Dean, D.A., Kusano-Kretzmer, K., Mayer, E.J., Rochester, D.E., Rogers, S.G., and Fraley, R.T. 1987. Insect tolerant transgenic tomato plants. Bio/Technology 5: 807–813.
Vaeck, M., Reynaerts, A., Hofte, H., Jansens, S., De Beuckeleen, M., Dean, C., Zabeau, M., Van Montagu, M., and Leemans, J. 1987. Transgenic plants protected from insect attack. Nature 328: 33–37.
Boulter, D., Gatehouse, A.M.R., and Hilder, V. 1989. Use of cowpea trypsin inhibitor (CpTI) to protect plants against insect predation. Biotechnol. Adv. 7: 489–497.
Ryan, C.A. 1990. Protease inhibitors in plants: Genes for improving defense against insects and pathogens. Annu. Rev. Phytopath. 28: 25–49.
Ryan, C.A. 1989. Proteinase inhibitor gene families: Strategies for transformation to improve plant defense against herbivores. BioEssays 10: 20–24.
Chrispeels, M.J. and Raikhel, N.V. 1991. tins, lectin genes and their role in plant defense. Plant Cell 3: 1–19.
Hilder, V.A., Powell, K.S., Gatehouse, A.M.R., Gatehouse, J.A., Gatehouse, L.N., Shi, Y., Hamilton, W.D.O., Merryweather, A., Newell, C.A., Timans, J.C., Peumans, W.J., van Damme, E., and Boulter, D. 1995. Expression of snowdrop lectin in transgenic tobacco plants results in added protection against aphids. Transgenic Res. 4: 18–25.
Schroeder, H.E., Gollasch, S., Moore, A., Tabe, L.M., Graig, S., Hardie, D.C., Chrispeels, M.J., Spencer, D., and Higgins, T.J.V. 1995. Bean α-amylase inhibitor confers resistance to the pea weevil (Bruchus pisorum) in transgenic peas (Pisum sativum L.). Plant Physiol. 107: 1233–1239.
Shade, R.E., Schroeder, H.E., Pueyo, J.J., Tabe, L.M., Murdock, L.L., Higgins, T.J.V., and Chrispeels, M.J. 1994. Transgenic pea seeds expressing the a-amylase inhibitor of the common bean are resistant to bruchid beetles. Bio/Technology 12: 793–796.
Masoud, S.A., Johnson, L.B., White, F.F., and Reeck, G.R. 1993. Expression of a cysteine proteinase inhibitor (oryzacystatin-l) in transgenic tobacco plants. Plant Mol. Biol. 21: 655–663.
Wolfson, J.L. and Murdock, L.L. 1990. Diversity in digestive proteinase activity among insects. J. Chem. Ecol. 16: 1089–1102.
Hilder, V.A., Gatehouse, A.M.R., Sheerman, S.E., Barker, R.F., and Boulter, D. 1987. A novel mechanism of insect resistance engineered into tobacco. Nature 330: 160–163.
Johnson, R., Narvaez, J., An, G., and Ryan, C. 1989. Expression of proteinase inhibitors I and II in transgenic tobacco plants: Effects on natural defense against Manduca sexta larvae. Proc. Natl. Acad. Sci. USA 86: 9871–9875.
Wu, R., Duan, X., and Xu, D. 1993. Analysis of rice genes in transgenic plants. Progress Nucleic Acid Res. Mol. Biol. 45: 1–26.
McElroy, D., Zhang, W., and Wu, R. 1990. Isolation of an efficient actin promoter for use in rice transformation. Plant Cell 2: 163–171.
Xu, D., McElory, D., Thornburg, R.W., and Wu, R. 1993. Systemic induction of a potato pin2 promoter by wounding, methyl jasmonate, and abscisic acid in transgenic rice plants. Plant Mol. Biol. 22: 573–588.
Xu, D., Wu, T., Cao, J., and Wu, R. 1993b. Production and analysis of transgenic rice plants, pp. 130–135. in Biotechnology in Agriculture. You, C. B., Chen, Z. L. and Ding, Y. (eds.). Kluwer Academic Publishers, The Netherlands.
Fujimoto, H., Itoh, K., Yamamoto, M., Kyozuka, J., and Shimamoto, K. 1993. Insect resistant rice generated by introduction of a modified δ-endotoxin gene of Bacillus thuringiensis . Bio/Technology 11: 1151–1155.
Hayakawa, T., Zhu, Y., Itoh, K., Kimura, Y., Izawa, T., Shimamoto, K., and Toriyama, S. 1992. Genetically engineered rice resistant to rice stripe virus, an insect-transmitted virus. Proc. Natl. Acad. Sci. USA 89: 9865–9869.
Cao, J., Duan, X., McElroy, D., and Wu, R. 1992. Regeneration of herbicide resistant transgenic rice plants following microprojectile-mediated transformation of suspension culture cells. Plant Cell Rep. 11: 586–591.
Kao, K.N. 1977. Chromosomal behavior in somatic hybrids of soybean-nicotiana glauca. Mol. Gen. Genet. 150: 225–230.
Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioas-says with tobacco tissue cultures. Physiol. Plant 15: 473–497.
Zhao, X., Wu, T., Xie, Y., and Wu, R. 1989. Genome-specific repetitive sequences in the genus of Oryza . Theor. Appl. Genet. 78: 201–209.
Bradford, M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal. Biochem. 72: 248–254.
Schwert, G.W. and Takenaka, Y. 1955. A spectrophotometric determination of trypsin and chymotrypsin. Biochim. Biophys. Acta 16: 570–575.
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Duan, X., Li, X., Xue, Q. et al. Transgenic rice plants harboring an introduced potato proteinase inhibitor II gene are insect resistant. Nat Biotechnol 14, 494–498 (1996). https://doi.org/10.1038/nbt0496-494
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DOI: https://doi.org/10.1038/nbt0496-494
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