Abstract
Tumor-associated carbohydrate (TAC) antigens are important targets in cancer vaccine efforts. Carbohydrates are, however, frequently poor immunogens, in that they are T-cell–independent antigens. Molecular mimicry of TAC by peptides is an alternative approach to generating anti-carbohydrate immune responses. Here we demonstrate that peptide mimotopes can elicit antibody responses that cross-react with representative human TAC antigens. Primary immunization with such a multiple antigenic peptide, along with QS-21 as adjuvant, elicits cytotoxic antibodies reactive with naturally occurring forms of TAC expressed on tumor cells, and vaccination of mice with peptide mimotopes reduced tumor growth and prolonged host survival in a murine tumor model.
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Acknowledgements
This work was supported by the USAMRMC (DAMD17-94-J-4310) Breast Cancer Program (T.K.E.) and NIH grant AI 45133 (T.K.E.). We thank Charlotte Read Kensil of Aquila Pharmaceuticals (Worcester, MA.) for supplying the QS-21. We also thank Dr. A. DeLeo (University of Pittsburgh) for the Meth A cell line and Dr. A Fulton (University of Maryland Cancer Center) for the murine breast lines.
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Kieber-Emmons, T., Luo, P., Qiu, J. et al. Vaccination with carbohydrate peptide mimotopes promotes anti-tumor responses . Nat Biotechnol 17, 660–665 (1999). https://doi.org/10.1038/10870
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DOI: https://doi.org/10.1038/10870
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