Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Specificity by design

Nature Biotechnology volume 24, pages 954–955 (2006)Cite this article

The specificity of a homing endonuclease has been altered using computational modeling of the protein-DNA interface.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Studies of the homing endonuclease I-MsoI (ref. 1) used detailed atomic level modeling to retarget the protein to an altered DNA site.

Katie Ris

References

  1. Ashworth, J. et al. Nature 441, 656–659 (2006).

    Article  CAS  Google Scholar 

  2. Stoddard, B.L. Q. Rev. Biophys. 38, 49–95 (2006).

    Article  Google Scholar 

  3. Havranek, J.J., Duarte, C.M. & Baker, D. J. Mol. Biol. 344, 59–70 (2004).

    Article  CAS  Google Scholar 

  4. Morozov, A.V., Havranek, J.J., Baker, D. & Siggia, E.D. Nucleic Acids Res. 33, 5781–5798 (2005).

    Article  CAS  Google Scholar 

  5. Pabo, C.O. & Sauer, R.T. Annu. Rev. Biochem. 61, 1053–1095 (1992).

    Article  CAS  Google Scholar 

  6. Pabo, C.O. & Nekludova, L. J. Mol. Biol. 301, 597–624 (2000).

    Article  CAS  Google Scholar 

  7. Wolfe, S.A., Nekludova, L. & Pabo, C.O. Annu. Rev. Biophys. Biomol. Struct. 3, 183–212 (1999).

    Google Scholar 

  8. Pabo, C.O., Peisach, E. & Grant, R.A. Annu. Rev. Biochem. 70, 313–340 (2001).

    Article  CAS  Google Scholar 

  9. Urnov, F.D. et al. Nature 435, 646–651 (2005).

    Article  CAS  Google Scholar 

  10. Bradley, P., Misura, K.M.S. & Baker, D. Science 309, 1868–1871 (2005).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. visiting professor in the Department of Systems Biology, Harvard Medical School, 200 Longwood Avenue, WAB 536, Boston, 02115, Massachusetts, USA

    Carl O Pabo

Authors
  1. Carl O Pabo
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pabo, C. Specificity by design. Nat Biotechnol 24, 954–955 (2006). https://doi.org/10.1038/nbt0806-954

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt0806-954

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing