Abstract
Longitudinal F-actin cables are thought to be important for transporting materials for polarized cell growth in fission yeast. We show that most F-actin in the cables is oriented such that the barbed end faces the nearest cell tip during interphase; however, this directionality is reversed during mitosis. These orientations of F-actin ensure proper transport of materials to growing sites during these cell-cycle stages.
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Acknowledgements
We thank J. Usukura and M. Sameshima for help in determining the directionality of F-actin. This study was supported by grants from the Ministry of Education, Science and Culture of Japan.
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Supplementary figures S1 and S2, supplementary table S1 and supplementary methods (PDF 344 kb)
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Kamasaki, T., Arai, R., Osumi, M. et al. Directionality of F-actin cables changes during the fission yeast cell cycle. Nat Cell Biol 7, 916–917 (2005). https://doi.org/10.1038/ncb1295
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DOI: https://doi.org/10.1038/ncb1295
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