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OsHAL3 mediates a new pathway in the light-regulated growth of rice

Nature Cell Biology volume 11, pages 845–851 (2009)Cite this article

Interestingly, OsHAL3-overexpression lines exposed to different light conditions showed a variety of phenotypes. OsHAL3-overexpressing plants grew faster than wild-type plants under normal white light/dark cycles (Fig. 1c) or blue light/dark cycles (Fig. 1d). However, such a growth-enhancing effect was not observed under continuous white light (Fig. 1f), blue light (Fig. 1g), darkness (Fig. 1h) or far-red light (Fig. 1i). A weak phenotype was observed under continuous red light conditions (Fig. 1e). These data suggest that OsHAL3-promoted growth is regulated by different light regimes.

A previous study showed that an oxidative environment facilitates the dissociation of HAL3, suggesting that the maintenance of the HAL3 trimer requires the reduced FMN ligand18. Given that light produces reactive oxygen species in plants cells, it is reasonable to propose that photo-oxidation may have caused the trimer disassembly in rice seedlings. This hypothesis is supported by the dissociation of the OsHAL3 trimer by blue, red and far-red light. However, blue light is the stronger inducer of OsHAL3 dissociation. This may suggest that blue light is more closely involved in the trimer disruption (Fig. 2b), because FMNH2 directly absorbs blue but not red or far-red light (Fig. 2d). Alternatively, dissociation may be mediated by blue light photoreceptors.

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Figure 1: The enhanced growth rate enabled by OsHAL3 overexpression is regulated by light.
Figure 2: Light-mediated OsHAL3 trimer dissociation.
Figure 3: Expression pattern of OsHAL3.
Figure 4: OsHAL3 function in growth regulation may involve ubiquitin-dependent proteolysis but independent from its PPC decarboxylase activity.

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NCBI Reference Sequence

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Acknowledgements

We thank Q..-H. Yao for providing a rice cDNA library and T. G. Sors for revising the manuscript. This work was supported by grants from the Ministry of Science and Technology of China (2006CB100100 and 2006AA10A102), the Chinese Academy of Sciences (KSCX2-YW-N-011), the National Natural Science Foundation of China (30730058 and 30821004), the Ministry of Agriculture of China (2009EX08009-0678) and the Shanghai Science and Technology Development Fund.

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  1. Shi-Yong Sun and Dai-Yin Chao: These authors contributed equally to this work.

Authors and Affiliations

  1. National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, 300 Fenglin Road, Shanghai, 200032, China

    Shi-Yong Sun, Dai-Yin Chao, Xin-Min Li, Min Shi, Ji-Ping Gao, Mei-Zhen Zhu, Hong-Quan Yang & Hong-Xuan Lin

  2. SIBS-UC Berkeley Center for Molecular Life Sciences, Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, China

    Sheng Luan & Hong-Xuan Lin

  3. Department of Plant and Microbial Biology, University of California, Berkeley, 94720, CA, USA

    Sheng Luan

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Contributions

H.X.L., D.Y.C and S.Y.S. designed the experiments; H.Q.Y. designed some experiments; S.Y.S. and D.Y.C. performed most of the experiments; H.X.L., X.M.L., S.M., J.P.G. and M.Z.Z. performed some of the experiments; H.X.L. conceived and supervised the work and D.Y.C, H.X.L. and S. L. wrote the manuscript.

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Correspondence to Hong-Xuan Lin.

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Sun, SY., Chao, DY., Li, XM. et al. OsHAL3 mediates a new pathway in the light-regulated growth of rice. Nat Cell Biol 11, 845–851 (2009). https://doi.org/10.1038/ncb1892

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