Supplementary Figure 3: Insulin-like signalling to the germline regulates developmental arrest in response to osmotic stress.

Percent of wild-type and gpdh-1(ok1558); gpdh-2(ok1733) animals developing past the L1 larval stage after 48 h. Error bars, s.d. n = 3 experiments of >100 animals. (b) Percent of wild-type and daf-2(e1370) animals failing to arrest development at 500 mM NaCl. The pie-1 promoter was used to drive DAF-2 expression specifically in the germline. Germline #1 and germline #2 represent two independently integrated transgenes. Error bars, s.d. n = 3 experiments of >100 animals. (c) Percent of wild-type and lin-45(n2018) animals developing past the L1 larval stage after 48 h. Error bars s.d. n = 3 experiments of >100 animals. (d) Percent of progeny from animals exposed to either DMSO or the MEK inhibitor U0126 dissolved in dimethyl sulfoxide (DMSO) developing past the L1 larval stage after 48 h (500 mM NaCl). Error bars s.d. n = 3 experiments of >100 animals. (e) Percent of progeny from animals exposed to either L4440 empty vector or mek-2 RNAi that developed past the L1 larval stage after 48 h (500 mM NaCl). Error bars s.d. n = 3 experiments of >100 animals. (f) Percent of rrf-1(pk1417) progeny, which exhibit reduced RNAi silencing in somatic tissue but retain germline RNAi silencing¹, from animals exposed to either L4440 empty vector or mek-2 RNAi that developed past the L1 larval stage after 48 h (500 mM NaCl). Error bars s.d. n = 3 experiments of >100 animals. The quantified results are presented as mean ± s.d. using ANOVA (b) and two-tailed t-test (d,e,f). ^∗∗P < 0.01,^∗∗∗P < 0.001, were considered significant. n.s., not significant. See Statistics Source Data in Supplementary Table 6.