Two papers provide insight into the reactivity of cytochrome P450s. A direct link between electron donation and reactivity has been shown with a selenocysteine-ligated P450 compound I, whereas a serine-ligated P450 (P411) has been engineered to catalyse an intermolecular C–H amination via nitrene transfer.
This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$29.99 / 30 days
cancel any time
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Ortiz de Montellano, P. R. & De Voss, J. J. in Cytochrome P450: Structure, Mechanism, and Biochemistry 4th edn (Ed. Ortiz de Montellano, P. R.) (Springer, 2015).
Denisov, I. G., Makris, T. M., Sligar, S. G. & Schlichting, I. Chem. Rev. 105, 2253–2277 (2005).
Fasan, R. ACS Catal. 2, 647–666 (2012).
Urlacher, V. B. & Girhard, M. Trends Biotechnol. 30, 26–36 (2012).
Onderko, E. L., Silakov, A., Yosca, T. H. & Green, M. T. Nat. Chem. 9, 623–628 (2017).
Prier, C. K., Zhang, R. K., Buller, A. R., Brinkmann-Chen, S. & Arnold, F. H. Nat. Chem. 9, 629–634 (2017).
Poulos, T. L. Chem. Rev. 114, 3919–3962 (2014).
Guengerich, F. P. & Munro, A. W. J. Biol. Chem. 288, 17065–17073 (2013).
Sigman, J. A., Pond, A. E., Dawson, J. H. & Lu, Y. Biochemistry 38, 11122–11129 (1999).
Adachi, S. et al. Biochemistry 32, 241–252 (1993).
Auclair, K., Moenne-Loccoz, P. & Ortiz de Montellano, P. R. J. Am. Chem. Soc. 123, 4877–4885 (2001).
Aldag, C. et al. Proc. Natl Acad. Sci. USA 106, 5481–5486 (2009).
Sivaramakrishnan, S. et al. Biochemistry 50, 3014–3024 (2011).
Rittle, J. & Green, M. T. Science 330, 933–937 (2010).
McIntosh, J. A. et al. Angew Chem. Int. Ed. 52, 9309–9312 (2013).
Singh, R., Kolev, J. N., Sutera, P. A. & Fasan, R. ACS Catal. 5, 1685–1691 (2015).
Prier, C. K., Hyster, T. K., Farwell, C. C., Huang, A. & Arnold, F. H. Angew Chem. Int. Ed. Eng. 55, 4711–4715 (2016).
Farewell, C. C., Zhang, R. K., McIntosh, J. A., Hyster, T. K. & Arnold, F. H. ACS Cent. Sci. 1, 89–93 (2015).
Sreenilayam, G., Moore, E. J., Steck, V. & Fasan, R. Adv. Synth. Cat. http://dx.doi.org/10.1002/adsc.201700202 (2017).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Fasan, R. New functional twists for P450s. Nature Chem 9, 609–611 (2017). https://doi.org/10.1038/nchem.2810
Published:
Issue Date:
DOI: https://doi.org/10.1038/nchem.2810
This article is cited by
-
Regio- and enantioselective O-demethylation of tetrahydroprotoberberines by cytochrome P450 enzyme system from Streptomyces griseus ATCC 13273
Applied Microbiology and Biotechnology (2019)