Figure 7: MML-1/MXL-2 preferentially regulated genes contribute to gonadal longevity.
From: Mondo complexes regulate TFEB via TOR inhibition to promote longevity in response to gonadal signals
(a) qRT–PCR analysis of MML-1/MXL-2 preferentially regulated genes after treatment with control or TOR RNAi. The bar represents mean±s.e.m. from three biological replicates relative to N2. P-value (*P<0.05 and **P<0.01) was determined by one-way analysis of variance (ANOVA) with Tukey’s test. (b) Knockdown of fat-5, mdl-1 and swt-1 significantly suppress glp-1 longevity. (c) Representative fluorescent images of MDL-1::GFP in indicated strains at day 1 adult. Upregulation of MDL-1::GFP in glp-1 background is abolished by mml-1 or mxl-2 deletion. (d) Quantification of MDL-1::GFP in the head region in indicated genotypes. Bars indicate mean±s.e.m. from 3 biological replicates (>20 worms each). P-value (***P<0.001) was determined by t-test. (e) MDL-1 overexpression extends wild-type lifespan. Scale bars, 50 μm (c).
